| 基于UPLC-MS/MS定量检测法优化灵芝三萜热回流提取工艺的研究 |
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| 引用本文： | 彭小芳,程池露,唐庆九,张劲松,唐传红,王晨光,冯娜,韩伟. 基于UPLC-MS/MS定量检测法优化灵芝三萜热回流提取工艺的研究[J]. 菌物学报, 2021, 40(9): 2480-2494. DOI: 10.13346/j.mycosystema.210137 |
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| 作者姓名： | 彭小芳 程池露 唐庆九 张劲松 唐传红 王晨光 冯娜 韩伟 |
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| 作者单位： | 1.华东理工大学药学院制药工程与过程化学教育部工程研究中心 上海市新药设计重点实验室 上海 2002372.上海市农业科学院食用菌研究所 国家食用菌工程技术研究中心 国家食用菌加工技术研发分中心 农业部南方食用菌资源利用重点实验室 上海市农业遗传育种重点开放实验室 上海 2014033.上海百信生物科技有限公司 上海 2014034.上海海洋大学食品学院 上海 201306 |
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| 基金项目： | 上海市科学技术委员会科研计划(17391903300) |
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| 摘 要： | 基于超高效液相-三重四级杆质谱联用（UPLC-MS/MS）定量检测法优化出一种灵芝三萜热回流提取的工艺。通过本实验建立的灵芝三萜UPLC-MS/MS精确方法检测不同来源的7个栽培品种灵芝子实体中19个三萜化合物的含量,筛选出三萜含量较高的sd-2灵芝子实体作为提取原料,以三萜含量和提取物得率为指标,通过单因素和响应面实验对灵芝三萜的热回流工艺进行优化,获得的最佳条件为：乙醇浓度75%、提取时间2.5h、液料比14:1、提取次数2次。对获得的最佳提取工艺进行中试实验,灵芝子实体乙醇提取物的得率为6.10%,三萜含量为11.8591mg/g。研究结果可为灵芝三萜的工业大规模提取提供理论依据,为灵芝高附加值产品的开发利用提供技术支撑。
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| 关 键 词： | 三萜类化合物 液质联用分析 中试提取 响应面优化 |
| 收稿时间： | 2021-04-06 |
Optimization of the hot reflux extraction process of Ganoderma lingzhi triterpenoids based on UPLC-MS/MS quantitative detection method |
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| PENG Xiao-Fang,CHENG Chi-Lu,TANG Qing-Jiu,ZHANG Jing-Song,TANG Chuan-Hong,WANG Chen-Guang,FENG Na,HAN Wei. Optimization of the hot reflux extraction process of Ganoderma lingzhi triterpenoids based on UPLC-MS/MS quantitative detection method[J]. Mycosystema, 2021, 40(9): 2480-2494. DOI: 10.13346/j.mycosystema.210137 |
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| Authors: | PENG Xiao-Fang CHENG Chi-Lu TANG Qing-Jiu ZHANG Jing-Song TANG Chuan-Hong WANG Chen-Guang FENG Na HAN Wei |
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| Abstract: | Hot reflux extraction process was optimized for Ganoderma lingzhi triterpenoids based on ultra-high performance liquid-triple quadrupole mass spectrometry (UPLC-MS/MS) quantitative detection method. The established UPLC-MS/MS method was used to precisely detect the content of 19 triterpenoids of G. lingzhi fruiting bodies of seven cultivated varieties from different sources, and fruiting body of sd-2 G. lingzhi with high triterpenoids content was selected as the extractive material. Based on triterpenoid content and extract yield, the triterpenoid extraction process was optimized through single factor and response surface experiments. The best optimization conditions obtained were as follows: 75% of ethanol concentration, 2.5h of extraction time, 14:1 of liquid-to-material ratio, and two times of repeated extraction. A pilot experiment was conducted for obtained optimal extraction technology, and the yield of the ethanol extract of G. lingzhi fruiting body was 6.10%, and the content of triterpenoid was 11.8591mg/g. The results provide theoretical reference for the industrialized large-scale extraction of G. lingzhi triterpenoids and technical support of further development and utilization of G. lingzhi high value-added products. |
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| Keywords: | triterpenoids LC-MS analysis pilot extraction response surface optimization |
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