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Purification of pseudouridylate synthetase I from Salmonella typhimurium. |
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| Authors: | F Arena G Ciliberto S Ciampi R Cortese |
| Abstract: | Pseudouridylate synthetase from Salmonella typhimurium has been purified 1,000 fold and is about 90% pure. The enzyme has a molecular weight of 50,000 daltons. In the presence of tRNA there is a change in molecular weight from 50.000 to 100.000. This change does not seem to be due to the formation of a tRNA-enzyme complex but rather to a tRNA induced dimerization. Other properties of the enzyme are described. |
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