Two-dimensional electrophoresis of plasma membranes, showing differences among wild-type and abnormal ascospore mutant strains of Neurospora crassa. |
| |
| Authors: | J B Nasrallah and A M Srb |
| |
| Abstract: | Plasma membranes isolated from vegetative cultures of wild-type Neurospora crassa were analyzed by two-dimensional electrophoresis, followed by staining with silver nitrate to visualize proteins and fluorescein-labeled concanavalin A to visualize glycosylated subunits. Mycelial plasma membranes from strains carrying mutations affecting ascospores were also analyzed. Two of the mutant strains were shown to have aberrant two-dimensional membrane subunit patterns. The correlation of these abnormalities with the known electron microscopic evidence for aberrations of their ascospore-delimiting membrane during ascospore genesis is discussed. |
| |
| Keywords: | |
|
|
