Respiration of Tritiated Substrates in Heterotrophic Activity Assays |
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| Authors: | Jorma Kuparinen and Timo Tamminen |
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| Abstract: | The respiration percentage of 3H-labeled glucose by heterotrophic microorganisms in water samples was determined by a method based on equimolar addition of [3H]- and [14C]glucose to samples. The respiration percentage of [3H]glucose exceeded that of [14C]glucose by 10% over the assayed range of respiration rates. Respiration percentages diminished with decreasing temperature, the relation between respiration of [3H]- and [14C]glucose remaining the same. The significance of tritiated water respiration with regard to experimental design is emphasized. |
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