| Factors Affecting Electro－Fusion and Electro－Activation In Serial Nuclear Transplantation In Goat（Carpa hircus） Embryo |
| |
| 引用本文： | 王玉阁,邹贤刚,徐少甫,杜淼.Factors Affecting Electro－Fusion and Electro－Activation In Serial Nuclear Transplantation In Goat（Carpa hircus） Embryo[J].基因组蛋白质组与生物信息学报,1994(2). |
| |
| 作者姓名： | 王玉阁 邹贤刚 徐少甫 杜淼 |
| |
| 作者单位： | WANG Yu-ge; ZOU Xian-gang;XU Shao-fu; DU Miao(Institute of Developmental Biology. Academia Sinica，Beijing 100080)(College of Agriculture. Yangzhou Universih，Yangzhou. Jiangsu. 225001) |
| |
| 摘 要： | ＦａｃｔｏｒｓＡｆｆｅｃｔｉｎｇＥｌｅｃｔｒｏ－ＦｕｓｉｏｎａｎｄＥｌｅｃｔｒｏ－ＡｃｔｉｖａｔｉｏｎＩｎＳｅｒｉａｌＮｕｃｌｅａｒＴｒａｎｓｐｌａｎｔａｔｉｏｎＩｎＧｏａｔ（Ｃａｒｐａｈｉｒｃｕｓ）ＥｍｂｒｙｏＷＡＮＧＹｕ－ｇｅ（王玉阁）；ＺＯＵＸ...
|
Factors Affecting Electro-Fusion and Electro-Activation in Serial Nuclear Transplantation in Goat(Carpa hircus) Embryo |
| |
| WANG Yu-ge, ZOU Xian-gang,XU Shao-fu, DU Miao.Factors Affecting Electro-Fusion and Electro-Activation in Serial Nuclear Transplantation in Goat(Carpa hircus) Embryo[J].Genomics Proteomics & Bioinformatics,1994(2). |
| |
| Authors: | WANG Yu-ge ZOU Xian-gang XU Shao-fu DU Miao |
| |
| Abstract: | The effects of the status of both donor and recipient cells (different maturity and different transplantation generations) on serial nuclear transplantation in goat were studied, using, as indices, electro-fusion rate, and the cleavage rate and development rate of the cultured embryos. As shown by the results,no significant difference in fusion rate was noted between donor cells of different maturity (recovered 26-28 hrs as against 32-36 hrs after LRH treatment ) and both types of donor cells responded to the given electrostimulation. With cells from ICM as donor, the fusion rate was lower than that using cells from 8-16 celled embryos and morula (G0: 0.010.05). The generation of transplantation produced no apparent effect on fusion rate. With the given electro-stimulation conditions, the average fusion rate was 88.58% fur GO and 89.38% for G1; average cleavage rate, 39.36%fur G0 and 47.75% for G1; and the proportion of embryos developing to morula and blastula was 15.17% for G0 and 18.2% fur G1. |
| |
| Keywords: | Goat Electro-fusion Electro-activation Serial nuclear transplantation |
| 本文献已被 CNKI 等数据库收录！ |
