用RAPD技术研究我国苹果果实轮纹病菌的遗传变异(英文)

为了从分子水平上明确近年来我国苹果生产上发生的A、B、D和F 4种不同症状型苹果果实轮纹病的遗传分化现象及其不同致病菌的遗传变异程度。本研究对来自我国三大苹果产区的26个相关病原菌株的DNA指纹图谱进行了分析(Table 1)。结果表明 (1) 上述4种不同症状型轮纹病原菌株在基因组DNA水平上具有明显的遗传差异(Fig.1&Table 2),并在所分析的11个多态性引物中有4个引物的扩增图谱(Table 4)均可使上述4种不同致病型菌株相互区别(Table 5)。统计分析结果显示,这4种不同致病菌相互之间的遗传距离为0.1314~0.2541(Table 6);(2) 相同症状型的不同来源致病菌之间具有较高的遗传相似性 (88.4~100%)(Table 3),由此说明苹果果实轮纹病菌的遗传分化与地理环境条件差异无明显的相关性。 以上研究结果对于进一步阐明苹果果实轮纹病害的遗传演化规律及该病害监测与防治技术的改进具有重要的理论与实际意义。

Study on the Genetic Variation of Physalospore piricola in Apple by Using RAPD Technique

In order to clarify the phenomenon of genetic variation in 4 different disease symptoms of apple disease, caused by Physalospore piriola, at molecular level, and the degree of genetic variation in 4 isolates of pathogenetic bacteria, the DNA fingerprint analysis of 26 related bacterial isolates of Ph. piricola from three major productive regions of apple in China was made by using RAPD technique. The results indicated that (1) Obvious genetic differences at the level of genomic DNA were found in different pathogenic bacterial isolate, and fingerprint of 4 of 11 polymorphic primers of amplification could distinguish the above mentioned 4 bacterial isolates from each other, among which the genetic distance was revealed by 0.1314~0.2541 on the basis of calculation of analytic data; (2) High genetic similarity (88.4~100%) between the identical symptom types from different sources of pathogenic bacteria were shown. It thus indicates the insignificant relation between the genetic differentiation and geographic difference. Above results of research are of importance in theoretic demonstration about the heredity and evolution of the disease Ph. piricola in apple fruit as well as in practice in improvement of identification and control technique for the disease.