| 热休克对于高粱细胞质雄性不育系线粒体数目及其育性的影响(英文) |
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| 引用本文： | 王俐,刘根齐,章银梅,王桂忱,祁新红,陈建南.热休克对于高粱细胞质雄性不育系线粒体数目及其育性的影响(英文)[J].基因组蛋白质组与生物信息学报,2000(2). |
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| 作者姓名： | 王俐 刘根齐 章银梅 王桂忱 祁新红 陈建南 |
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| 作者单位： | 中国科学院遗传研究所!中国北京100101(王俐，刘根齐)，锦州医学院!中国锦州121004(章银梅，王桂忱，祁新红，陈建南) |
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| 基金项目： | This work is supported by the National Natural Science Foundation of China! (Grant No. 39570397). |
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| 摘 要： | 对高梁雄性不育系 ３ １９７Ａ（３Ａ）及其保持系 ３ １９７Ｂ（３Ｂ）均分别进行热激处理，以常温处理为对照，以处于花粉母细胞期的小穗为材料提取其线粒体，首次采用流式细胞仪测量线粒体的数量。首次观察到：经热激处理后，不育系 ３Ａ线粒体的数目增加约 ３．５倍；相反，保持系３Ｂ在常温时，线粒体数量为３Ａ的３．５５倍，热激后却无明显变化（Ｆｉｇ．１）。同时取热激处理及常温下３Ａ及３Ｂ的花药淀粉粒作碘化钾染色，结果表明：热激后３Ａ花药中出现饱满淀粉粒（Ｆｉｇ．２）；对其细胞色素氧化酶和过氧物酶同功酶的研究表明，热激后３Ａ电泳酶谱中有新条带出现，而且与３Ｂ的带型一致（Ｆｉｇ．３）。
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Effects on Mitochondrion Numbers and the Fertility Through Heat Shock in CMS Sorghum |
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| WANG Li,LIU Gen-qi,ZHANG Yin-mei,WANG Gui-chen,QI Xin-hong and CHEN Jian-nan.Effects on Mitochondrion Numbers and the Fertility Through Heat Shock in CMS Sorghum[J].Genomics Proteomics & Bioinformatics,2000(2). |
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| Authors: | WANG Li LIU Gen-qi ZHANG Yin-mei WANG Gui-chen QI Xin-hong and CHEN Jian-nan |
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| Abstract: | The mitochondria of anther at pollen mother cell stage of cytoplasmic male sterile (CMS) line 3197A in Sorghum were extracted after heat shock. The number of mitochondria was measured with now cytometen and the isoenzymes in mitochondria were analyzed by PAGE combined with staining for enzyme activity. The result of quantity measurement of mitochondria showed that the number of mitochondria in CMS line 3197A increased greatly up to 4.5 folds after heat shock. The analysis of isoenzymes also demonstrated that the type of isoenzymes in CMS line was in accordance with the maintainer line. The study first showed the change of the quantity of mitochondria after heat shock and the relationship between this change and discussed the fertility in sorghum. |
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| Keywords: | mitochondrion now cytometer cytoplasmic male sterile (CMS) HS |
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