| The Epitope Study on the SARS-CoV Nucleocapsid Protein |
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| 作者姓名： | Li S Lin L Wang H Yin J Ren Y Zhao Z Wen J Zhou C Zhang X Li X Wang J Zhou Z Liu J Shao J Lei T Fang J Xu N Liu S |
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| 作者单位： | Shuting Li，Liang Lin，Hao Wang，Jianning Yin，Yan Ren，Zhe Zhao，Jie Wen，Cuiqi Zhou，Xumin Zhang，Xiaolei Li，Jingqiang Wang，Zhengfeng Zhou，Jinxiu Liu，Jianmin Shao，Tingting Lei，Jianqiu Fang，Ningzhi Xu，and Siqi Liu
Beijing Genomics Institute，Chinese Academy of Sciences，Beijing 101300，China & Beijing Proteomics Institute，Beijing 101300，China |
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| 摘 要： | The nucleocapsid protein (N protein) has been found to be an antigenic protein in a number of coronaviruses. Whether the N protein in severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is antigenic remains to be elucidated. Using Western blot and Enzyme-linked Immunosorbent Assay (ELISA), the recombinant N proteins and the synthesized peptides derived from the N protein were screened in sera from SARS patients. All patient sera in this study displayed strong positive immunoreactivities against the recombinant N proteins, whereas normal sera gave negative immunoresponses to these proteins, indicating that the N protein of SARS-CoV is an antigenic protein. Furthermore, the epitope sites in the N protein were determined by competition experiments, in which the recombinant proteins or the synthesized peptides competed against the SARS-CoV proteins to bind to the antibodies raised in SARS sera. One epitope site located at the C-terminus was confirmed as the most antigenic region in this prot
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The epitope study on the SARS-CoV nucleocapsid protein |
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| Li S,Lin L,Wang H,Yin J,Ren Y,Zhao Z,Wen J,Zhou C,Zhang X,Li X,Wang J,Zhou Z,Liu J,Shao J,Lei T,Fang J,Xu N,Liu S.The Epitope Study on the SARS-CoV Nucleocapsid Protein[J].Genomics Proteomics & Bioinformatics,2003,1(3):198-206. |
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| Authors: | Li Shuting Lin Liang Wang Hao Yin Jianning Ren Yan Zhao Zhe Wen Jie Zhou Cuiqi Zhang Xumin Li Xiaolei Wang Jingqiang Zhou Zhengfeng Liu Jinxiu Shao Jianmin Lei Tingting Fang Jianqiu Xu Ningzhi Liu Siqi |
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| Institution: | Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China. |
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| Abstract: | The nucleocapsid protein (N protein) has been found to be an antigenic protein in a number of coronaviruses. Whether the N protein in severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is antigenic remains to be elucidated. Using Western blot and Enzyme-linked Immunosorbent Assay (ELISA), the recombinant N proteins and the synthesized peptides derived from the N protein were screened in sera from SARS patients. All patient sera in this study displayed strong positive immunoreactivities against the recombinant N proteins, whereas normal sera gave negative immunoresponses to these proteins, indicating that the N protein of SARS-CoV is an antigenic protein. Furthermore, the epitope sites in the N protein were determined by competition experiments, in which the recombinant proteins or the synthesized peptides competed against the SARS-CoV proteins to bind to the antibodies raised in SARS sera. One epitope site located at the C-terminus was confirmed as the most antigenic region in this protein. A detailed screening of peptide with ELISA demonstrated that the amino sequence from Codons 371 to 407 was the epitope site at the C-terminus of the N protein. Understanding of the epitope sites could be very significant for developing an effective diagnostic approach to SARS. |
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| Keywords: | SARS coronavirus nucleocapsid protein antigenicity epitope |
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