双原核注射提高转基因的整合效率(英文)

本文提出了受精卵双原核注射方法，利用共注射具有部分同源区的两个片段鼠乳消酸蛋白基因 (WAP) 调控序列控制下的人粒细胞集落刺激因子 (G-CSF) (Fig.1)，通过PCR和Southern Blot检测了8细胞期外源基因的整合(Fig.2)。结果表明，与单一原核注射相比较，双原核注射转基因的整合率由单一原核注射的48.68%提高至62.65%(Table 1)。这为转基因动物建立，提高外源基因整合率提供了新途径。

Dual Pronuclear Co-injection to Raise Transgenic Integration Efficiency

The method of dual pronuclear co-injection into fertilized egg was put forward. Identification of integration of exogenous genes from the pronuclei of 8-celled phase which were controlled under the regulation sequence of human granulocyte colony-stimulating factor (G-CSF) by using co-injection of two fragments of whey acidic protein (WAP) which have part of homologous domain, was conducted through PCR and Southern blotting techniques. The results indicated that the integration efficiency resulting from dual pronuclear injection revealed an increase by 62.65%, comparing to that from single injection, 48.68%. It provides a new pathway for raising integration efficiency in setting up transgenic animals.