| In situ Localization and Isolation of Actin Filaments from Pollen Tubes of Amaryllis vittata Ait |
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| 引用本文： | 蔡雪,董云洲.In situ Localization and Isolation of Actin Filaments from Pollen Tubes of Amaryllis vittata Ait[J].基因组蛋白质组与生物信息学报,1994(2). |
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| 作者姓名： | 蔡雪 董云洲 |
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| 作者单位： | CAl Xue;DONG Yun-zhou(College of Life Sciences，Peking University，Beijing100871)(Biotechnology Research Center，Chinese Academy of Agricultural Sciences，Beijing100081) |
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| 摘 要： | ＩｎｓｉｔｕＬｏｃａｌｉｚａｔｉｏｎａｎｄＩｓｏｌａｔｉｏｎｏｆＡｃｔｉｎＦｉｌａｍｅｎｔｓｆｒｏｍＰｏｌｌｅｎＴｕｂｅｓｏｆＡｍａｒｙｌｌｉｓｖｉｔｔａｔａＡｉｔＣＡｌＸｕｅ（蔡雪）；ＤＯＮＧＹｕｎ－ｚｈｏｕ（董云洲）（ＣｏｌｌｅｇｅｏｆＬｉｆｅＳ...
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| 关 键 词： | Actin filament Pollen tube TRITC-phalloidin Cytochalasin D Isolation Mg￣(++) Amaryllis vittata Ait. |
In situ Localization and Isolation of Actin Filaments from Pollen Tubes of Amaryllis vittata Ait |
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| CAl Xue,DONG Yun-zhou.In situ Localization and Isolation of Actin Filaments from Pollen Tubes of Amaryllis vittata Ait[J].Genomics Proteomics & Bioinformatics,1994(2). |
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| Authors: | CAl Xue DONG Yun-zhou |
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| Abstract: | Actin filaments (AFs) in un-fixed pollen tubes of Amaryllis vittata Ait were visualized after TRITC-phalloidin staining with DMSO as a permeabilising agent. Typically, strands or hundles of microfilaments (Mfs) were distributed in the extreme tip as well as pollen tubes in a form of network.Fluorescent granules or circles of various sizes were frequently found that continued with the filamentous structures. In addition, a more brightly stained structure, possibly Mf organizing center, was observed. Treatment of pollen tubes with cytochalasin D(CD)for increasing time intervals (5-40 minutes) caused gradual reduction of strands until flurescent granules filled up the pollen tubes. Mcanwhile, cytoplasmie streaming was inhibited completely. Though closely associated with vegetative nuclei (VN) and generative cells (GC), AFs were not found in the cytoplasm of GC.Mg++concentration greatly affected the isolated Mfs. |
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