烟草花粉发育过程中的细胞凋亡及其与钙/钙调素依赖性蛋白激酶T1表达的关系(英文)

用原位末端标记及免疫组化显色方法，对烟草花药发育过程中的细胞凋亡进行了检测。结果表明绒毡层、环形细胞、药隔组织细胞在减数分裂后四分体时期开始凋亡(Plate I, C &D)，而花药维管束细胞从小孢子母细胞时期就开始凋亡(Plate I, E)。这些特定的细胞在花药发育特定时期的凋亡担负着特定的作用。钙/钙调素依赖性蛋白激酶T1在这些凋亡细胞中大量特异表达(Plate II)，表明钙信号途径参与了烟草花粉发育的细胞凋亡调控。

Pollination-induced Apoptosis in Tobacco Related to Expression of Calcium/calmodulin-dependent Protein Kinase T1

Using the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) and immunohistochemical methods, the pollination-induced cell death in tobacco (Nicotiana tabacum L.) was detected. The results indicated that pollination induced apoptotic death in specific cells. Apoptosis was initiated during the tetrad stage after meiosis in the tapetal cells, cyclic cells, and connective cells (Plate I, C and D), and during pollen mother cell stage in the vascular bundle (Plate I, E). Those apoptotic events, which occurred in the specific cells in the specific stages of anther development, play some vital roles of specific biological function. The highly expression of calcium/calmodulin-dependent protein kinase T1 related closely to these apoptotic processes (Plate II) in these cells, suggesting that calcium-mediated signal pathway is involved in the regulation of apoptosis during anther development in tobacco.