| Genetic Engineering of Tobacco with Double Resistance to Both Virus and Insect |
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| 引用本文： | 梁晓友,米景九,潘乃隧,陈章良.Genetic Engineering of Tobacco with Double Resistance to Both Virus and Insect[J].基因组蛋白质组与生物信息学报,1994(1). |
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| 作者姓名： | 梁晓友 米景九 潘乃隧 陈章良 |
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| 作者单位： | National Laboratory of Plant Geiietic Engjneenng Department of Biology，Peking University，100871. Laboratory of Molecular Geiietics，Biological College，Beijing Agricultural University，100094. Deb |
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| 摘 要： | ＧｅｎｅｔｉｃＥｎｇｉｎｅｅｒｉｎｇｏｆＴｏｂａｃｃｏｗｉｔｈＤｏｕｂｌｅＲｅｓｉｓｔａｎｃｅｔｏＢｏｔｈＶｉｒｕｓａｎｄＩｎｓｅｃｔＬＩＡＮＧＸｉａｏ－ｙｏｕ；（梁晓友）ＭＩＪｉｎｇ－ｊｉｕ；（米景九），ＰａｎＮａｉ－ｓｕｉ（潘乃隧），ＣＨＥＮｚｈ...
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Genetic Engineering of Tobacco with Double Resistance to Both Virus and Insect |
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| LIANG Xiao-you, MI Jing-jiu,Pan Nai-sui,CHEN zhang-liang.Genetic Engineering of Tobacco with Double Resistance to Both Virus and Insect[J].Genomics Proteomics & Bioinformatics,1994(1). |
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| Authors: | LIANG Xiao-you MI Jing-jiu Pan Nai-sui CHEN zhang-liang |
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| Abstract: | Expression vector pE14 with double resistance to virus and insect was constructed by inserting CW-cp gene and Bt-toxin gene one by into T-DNA of the same binary vector pE3. Tobacco was then transformed with Agrbacterium tumefaciens (At)GV311-SE carrying PE14. Nopaline assay and PCR amplification confirmed that both CW-cp gene and Bt-toxin gene had been introduced into tobacco genome by T-DNA of PE3. Test attack with virus and demonstrated, in some of the transgenic plants, double resistance to both infection by CMV and damage by Manduca sexta. |
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| Keywords: | Coiistruction of vector Transformation of tobacco Double resistance to virus and insect |
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