| Sperm Mediated Transfer of Genes into Goldfish and Detection by Polymerase Chain Reaction |
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| 作者姓名： | 于建康 阎维 张玉廉 费云标 黄涛 严绍颐 |
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| 作者单位： | Institute of Developmental Biology Academia Sinica.Beijing，100080，Institute of Developmental Biology，Academia Sinica.Beijing，100080，Institute of Developmental Biology，Academia Sinica.Beijing，100080，Institute of Developmental Biology，Academia Sinica.Beijing，100080，Institute of Developmental Biology，Academia Sinica.Beijing，100080，Institute of Developmental Biology，Academia Sinica.Beijing，100080 |
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| 摘 要： | Goldfish sperms were mixed with eggs for fertilization after incubation with antifreeze protein gene(AFP)from ocean pout for 30 min.A number of embryos and 145 adult goldfish were obtained.DNA from adult goldfish and embryos was extracted separately.Results of the amplification by PCRand Southern blot molecular hybridization indicate the integration of exogenous antifreeze gene intothe genome of a part of the recipient goldfish.Of the 45 samples detected by PCR,twelve showedpositive reaction with distinct hybridization band.The positive rate was 26%.
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Sperm Mediated Transfer of Genes into Goldfish and Detection by Polymerase Chain Reaction |
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| Yu Jian-Kang Yan Wei Zhang Yu-Lian Fei Yun-Biao Huang Tao Yan Shao-Yi Institute of Developmentl Biology,Academia Sinica.Sperm Mediated Transfer of Genes into Goldfish and Detection by Polymerase Chain Reaction[J].Genomics Proteomics & Bioinformatics,1992(1). |
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| Authors: | Yu Jian-Kang Yan Wei Zhang Yu-Lian Fei Yun-Biao Huang Tao Yan Shao-Yi Institute of Developmentl Biology Academia Sinica |
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| Institution: | Yu Jian-Kang Yan Wei Zhang Yu-Lian Fei Yun-Biao Huang Tao Yan Shao-Yi Institute of Developmentl Biology,Academia Sinica.Beijing,100080 |
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| Abstract: | Goldfish sperms were mixed with eggs for fertilization after incubation with antifreeze protein gene (AFP)from ocean pout for 30 min.A number of embryos and 145 adult goldfish were obtained. DNA from adult goldfish and embryos was extracted separately.Results of the amplification by PCR and Southern blot molecular hybridization indicate the integration of exogenous antifreeze gene into the genome of a part of the recipient goldfish.Of the 45 samples detected by PCR,twelve showed positive reaction with distinct hybridization band.The positive rate was 26%. |
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| Keywords: | Transgenic fish Polymerase chain reaction(PCR) |
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