Molecular characterization of the duck enteritis virus <Emphasis Type="Italic">UL4</Emphasis> gene |
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Authors: | Hua-qi Pan Nan Wang Li Liu Lei Liu Jiang-chun Hu Pu-yan Chen Shu-jin Wang Rui-bing Cao |
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Institution: | (1) Key Laboratory of Animal Disease Diagnosis and Immunology, Nanjing Agricultural University, Ministry of Agriculture, Nanjing, 210095, China;(2) Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, 110016, China;(3) College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, 730070, China |
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Abstract: | Duck enteritis virus (DEV) is a herpesvirus that causes an acute, contagious and fatal disease. In the present article, the
DEV UL4 gene was cloned and sequenced from a vaccine virus. A degenerate oligonucleotide primer for the consensus site of herpesvirus
UL3 gene and a specific primer located in UL5 were used in the polymerase chain reaction (PCR) to amplify a DNA product 2 086
bp in size. DNA sequence analysis revealed that a 714 bp open reading frame (ORF) of DEV encoding a 237 amino acid polypeptide
is homologous to the family of herpesvirus UL4 proteins and therefore has been characterized as a DEV UL4 gene. Alignment of the DEV UL4 protein sequence with those of other alphaherpesviruses showed that 10 amino acid residues
are completely conserved. Phylogenetic tree analysis showed that the seventeen alphaherpesviruses viruses analyzed were classified
into four large groups, and the duck enteritis virus branched separately, closely related to the Mardiviruses group comprising
Gallid herpesvirus 2 (GaHV-2), Gallid herpesvirus 3 (GaHV-3) and Meleagrid herpesvirus 1 (MeHV-1). The present study showed
that the evolutionary relationship of the UL4 protein could be used for classification of alphaherpesviruses.
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Keywords: | Duck enteritis virus (DEV) Degenerate primer PCR UL4 gene Gene Character |
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