Phosphatidylserine-Specific Phospholipase A1 is the Critical Bridge for Hepatitis C Virus Assembly |
| |
Authors: | Qi Yang Min Guo Yuan Zhou Xue Hu Yun Wang Chunchen Wu Min Yang Rongjuan Pei Xinwen Chen Jizheng Chen |
| |
Institution: | 1.Department of Gastroenterology, Guangzhou Women and Children's Medical Center, Guangzhou 510623, China2.State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China3.School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China |
| |
Abstract: | The phosphatidylserine-specific phospholipase A1(PLA1 A) is an essential host factor in hepatitis C virus(HCV)assembly. In this study, we mapped the E2, NS2 and NS5 A involved in PLA1 A interaction to their lumenal domains and membranous parts, through which they form oligomeric protein complexes to participate in HCV assembly. Multiple regions of PLA1 A were involved in their interaction and complex formation. Furthermore, the results represented structures with PLA1 A and E2 in closer proximity than NS2 and NS5 A, and strongly suggest PLA1 A-E2's physical interaction in cells. Meanwhile, we mapped the NS5 A sequence which participated in PLA1 A interaction with the C-terminus of domain 1. Interestingly, these amino acids in the sequence are also essential for viral RNA replication. Further experiments revealed that these four proteins interact with each other. Moreover, PLA1 A expression levels were elevated in livers from HCV-infected patients. In conclusion, we exposed the structural determinants of PLA1 A, E2, NS2 and NS5 A proteins which were important for HCV assembly and provided a detailed characterization of PLA1 A in HCV assembly. |
| |
Keywords: | Phosphatidylserine-specific phospholipase A1 (PLA1A) HCV assembly Viral RNA replication |
本文献已被 CNKI 等数据库收录! |
| 点击此处可从《中国病毒学》浏览原始摘要信息 |
| 点击此处可从《中国病毒学》下载免费的PDF全文 |
|