| 汉滩病毒S片段基因编码区在Vero-E6细胞中的表达 |
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| 引用本文： | 张梦华,王航雁,杨为松,黄长形,李光玉,汪毅,白雪帆.汉滩病毒S片段基因编码区在Vero-E6细胞中的表达[J].中国病毒学,1999,14(2):181-184. |
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| 作者姓名： | 张梦华 王航雁 杨为松 黄长形 李光玉 汪毅 白雪帆 |
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| 作者单位： | 第四军医大学唐都医院传染科,西安,710038 |
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| 摘 要： |
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| 关 键 词： | 汉滩病毒 基因表达 S片段 编码区 |
Transient Expression of Hantaan Virus S segment Gene Coding region in vitro of Vero-E6 cells |
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| Zhang Menghua,Wang Hangyan,Yang Weisong,Huang Changxing,Li Guangyu,Wang Yi,Bai Xuefan.Transient Expression of Hantaan Virus S segment Gene Coding region in vitro of Vero-E6 cells[J].Virologica Sinica,1999,14(2):181-184. |
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| Authors: | Zhang Menghua Wang Hangyan Yang Weisong Huang Changxing Li Guangyu Wang Yi Bai Xuefan |
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| Institution: | 1.Tangdu Hospital，The Fourth Military Medical University，Xi'an 710038 |
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| Abstract: | The coding region of S genome segment of Hantaan virus (76/118 strain) was inserted into the eukarytic expression plasmidpVR1012. The recombinant expression plasmid pVRS22 was constructed. Vero-E6 cells were transiently transfected in vitro with pVRS22 plasmid. The transient expression of Hantaan virus nucleocapsid proteins in Vero-E6 cells was detected by indirect immunofluorescence assay (IFA) with monoclonal antibody 5H5 against Hantaan virus. |
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| Keywords: | Hantaan virus Gene expression S segment Coding region |
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