Labeling of influenza viruses with synthetic fluorescent and biotin-labeled lipids |
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Authors: | Natalia A Ilyushina Evgeny S Chernyy Elena Y Korchagina Aleksandra S Gambaryan Stephen M Henry Nicolai V Bovin |
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Institution: | 1. Food and Drug Administration, 29 Lincoln Drive, Bethesda, MD, 20892, USA 2. Shemyakin Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho Maklaya 16/10, Moscow, 117997, Russia 3. Chumakov Institute of Poliomyelitis and Viral Encephalitides, Russian Academy of Medical Sciences, Moscow Region, 142782, Russia 4. Biotechnology Research Institute, AUT University, Private Bag 92006, Auckland, 1142, New Zealand
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Abstract: | Direct labeling of virus particles is a powerful tool for the visualization of virus-cell interaction events. However, this technique involves the chemical modification of viral proteins that affects viral biological properties. Here we describe an alternative approach of influenza virus labeling that utilizes Function-Spacer-Lipid (FSL) constructs that can be gently inserted into the virus membrane. We assessed whether labeling with fluorescent (fluo-Ad-DOPE) or biotin-labeled (biot-CMG2-DOPE) probes has any deleterious effect on influenza virus hemagglutinin (HA) receptor specificity, neuraminidase (NA) activity, or replicative ability in vitro. Our data clearly show that neither construct significantly affected influenza virus infectivity or viral affinity to sialyl receptors. Neither construct influenced the NA activities of the influenza viruses tested, except the A/Puerto Rico/8/34 (H1N1) strain. Our data indicate that lipid labeling provides a powerful tool to analyze influenza virus infection in vitro. |
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Keywords: | bioimaging Function-Spacer-Lipid (FSL) constructs labeling of influenza viruses synthetic lipids |
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