| 禽白血病病毒p19基因末端片段在大肠杆菌中的表达 |
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| 引用本文： | 刘公平,赵振芬,刘福安.禽白血病病毒p19基因末端片段在大肠杆菌中的表达[J].中国病毒学,2001,16(1):78-80. |
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| 作者姓名： | 刘公平 赵振芬 刘福安 |
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| 作者单位： | 1. 华南农业大学动物医学系,
2. 郑州牧业工程高等专科学校, |
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| 基金项目： | 高等学校博士学科点专项基金资助项目（980501） |
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| 摘 要： | 根据禽白血病病毒（ALV）p19基因末端序列合成一条82
bp的双链DNA片段，将其克隆到表达质粒pGEMEX-1中，序列分析结果与设计的相符。重组表达质粒转化的大肠杆菌BL21（DE3）经IPTG诱导后产生34kD融合表达产物，与理论值相符；Western-blot分析表明该表达产物能与兔抗ALV血清发生反应。
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| 关 键 词： | 禽白血病病毒 p19基因 克隆 原核表达 |
| 文章编号： | 1003-5125(2001)01-0078-03 |
| 修稿时间： | 2000年1月24日 |
Expression of p19 Gene of
Avian Leukosis Virus in Escherichia coli |
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| LIU Gong-ping,ZHAO Zhen-fen,LIU Fu-an.Expression of p19 Gene of
Avian Leukosis Virus in Escherichia coli[J].Virologica Sinica,2001,16(1):78-80. |
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| Authors: | LIU Gong-ping ZHAO Zhen-fen LIU Fu-an |
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| Abstract: | Based on avian leukosis virus ( ALV) p19 gene terminal nucleotide sequence, a 82 bp double-stranded DNA fragment was chemically synthesized and cloned into the expression vector pGEMEX-1. The sequencing result indicated th at the cloned fragment was a correct version of the one designed both in nucleot ide sequence and in its open reading frame. The recombinant was used to transfor m E.coli BL21 (DE3). The cloned fragment was expressed as a fused protein wi th T7 gene 10 leader peptide and was shown to be 34 kD in size on SDS-PAGE gel when induced with 1 mmol/L IPTG. The expression product was able to bind immunol ogically to rabbit anti-ALV serum in Western-blot assay and is being tested to differentiate exogenous from endogenous ALV. |
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