| 跨种扩增筛选大鳄龟微卫星标记及遗传多样性分析 |
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| 引用本文： | 闵金金,张加勇,郑荣泉,包得超.跨种扩增筛选大鳄龟微卫星标记及遗传多样性分析[J].动物学杂志,2013,48(6):926-932. |
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| 作者姓名： | 闵金金 张加勇 郑荣泉 包得超 |
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| 作者单位： | 浙江师范大学生态研究所 金华 321004;浙江师范大学特种水产研究所 金华 321004;浙江师范大学生态研究所 金华 321004;浙江师范大学生态研究所 金华 321004;浙江师范大学特种水产研究所 金华 321004;浙江师范大学化学与生命科学学院 金华 321004 |
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| 基金项目： | 浙江省“十二五”水产育种重大专项（No.2012C12907-9），浙江省重点科技创新团队自主确定项目 （No.2010R50026-07），浙江省大学生科技创新活动计划资助项目（No.2012R404068），金华市科技局重点项目（No.2010-2-012 ） |
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| 摘 要： | 跨种扩增是一种能够快速、有效地获得物种微卫星标记的方法。本研究利用在近缘种中已发表的微卫星DNA引物，对大鳄龟（Macroclemys temminckii）进行跨种PCR扩增，在合成的69对引物中获得8对具有多态性的微卫星位点。对PCR扩增产物进行统计，得出观测杂合度（Ho）的范围是0.041 7~0.954 5，平均为0.384 8；期望杂合度（HE）的范围为0.041 7~0.811 8，平均为0.479 1；多态信息含量范围为0.040 0~0.759 2，平均为0.423 2；经过卡方检验后，部分微卫星位点符合哈迪-温伯格平衡。总体来说，这些位点是研究大鳄龟遗传结构的良好分子标记。
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| 关 键 词： | 大鳄龟 微卫星标记 跨种扩增 遗传多样性 |
| 收稿时间： | 2013/1/21 0:00:00 |
| 修稿时间： | 2013/5/15 0:00:00 |
Isolation of Microsatellite Markers by Cross-amplification and Genetic Diversity Analysis in Macroclemys temminckii |
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| MIN Jin-Jin,ZHANG Jia-Yong,ZHENG Rong-Quan and BAO De-Chao.Isolation of Microsatellite Markers by Cross-amplification and Genetic Diversity Analysis in Macroclemys temminckii[J].Chinese Journal of Zoology,2013,48(6):926-932. |
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| Authors: | MIN Jin-Jin ZHANG Jia-Yong ZHENG Rong-Quan and BAO De-Chao |
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| Institution: | Institute of Ecology, Zhejiang Normal University, Jinhua 321004;Institute of Special Aquaculture Source, Zhejiang Normal University, Jinhua 321004;Institute of Ecology, Zhejiang Normal University, Jinhua 321004;Institute of Ecology, Zhejiang Normal University, Jinhua 321004;Institute of Special Aquaculture Source, Zhejiang Normal University, Jinhua 321004;College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China |
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| Abstract: | The method of cross-amplification can be used to obtain microsatellite marker quickly and effectively. This study amplified microsatellite loci of Macroclemys temminckii using published microsatellite DNA primers from related species. In M.temminckii, 8 microsatellite loci which had polymorphism were found in 69 microsatellite markers. By analyzing PCR amplification products, a total of 30 alleles were detected, and the average number of alleles was 3.75. The observed heterozygosity (Ho) ranged from 0.041 7 to 0.954 5, and expected heterozygosity (HE) was 0.041 7 to 0.811 8. PIC value was from 0.040 0 to 0.759 2, averaging 0.423 2. After chi-square test, some loci were found to correspond to Hardy-Weinberg equilibrium. In general, these loci are good molecular markers that can be used for studying genetic structure for M.temminckii. |
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| Keywords: | Macroclemys temminckii Microsatellite marker Cross-amplification Genetic diversity |
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