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| 中国南方海域4种石斑鱼的遗传多样性及分子系统发生关系的分析 | |
| 引用本文： | 吕锋骅,韩洁,董颖,蔡厚才.中国南方海域4种石斑鱼的遗传多样性及分子系统发生关系的分析[J].动物学杂志,2010,45(6):9-18. |
| 作者姓名： | 吕锋骅 韩洁 董颖 蔡厚才 |
| 作者单位： | 1. 生物多样性与生态工程教育部重点实验室,北京师范大学生命科学学院,北京,100875 2. 生物多样性与生态工程教育部重点实验室,北京师范大学生命科学学院,北京,100875;北京市水产科学研究所,北京,100068 3. 南麂列岛国家级海洋自然保护区,温州,325014 |
| 基金项目： | 浙江省温州市科技局研究项目(No.S20070055); 北京师范大学博士学位论文培育基金项目(No.10420801) |
| 摘 要： | 对中国南方海域的青石斑鱼(Epinephelus awoara)、赤点石斑鱼(E.akaara)、橙点石斑鱼(E.bleekeri)及蜂巢石斑鱼(E.merra)进行了遗传多样性和分子系统发生关系的分析。对9个微卫星DNA标记的基因型测定结果进行计算,发现这4个地理分布重叠且形态相似的石斑鱼均具有较丰富的遗传多样性,其平均等位基因数、平均多态信息含量(PIC)、平均观测杂合度(Ho)及平均期望杂合度(He)的取值范围分别为(8.22±5.02)～(18.67±9.38)、(0.56±0.21)～(0.83±0.13)、(0.62±0.03)～(0.85±0.02)和(0.60±0.07)～(0.86±0.04)。经哈迪-温伯格平衡(HWE)检验,除橙点石斑鱼外,其他3种鱼的微卫星DNA位点都基本符合平衡。利用微卫星DNA标记和线粒体细胞色素b基因对这4种鱼重建的分子系统树均显示,青石斑鱼与赤点石斑鱼的亲缘关系较近。以微卫星DNA标记对所研究石斑鱼的全部个体进行贝叶斯聚类分析的结果表明,4个鱼物种之间的分化关系明确。 |
| 关 键 词： | 石斑鱼 微卫星 细胞色素b基因 遗传多样性 分子系统发生 |
| 收稿时间： | 2010/4/30 0:00:00 |
| 修稿时间： | 9/2/2010 12:00:00 AM |
Genetic Diversity and Phylogenetic Relationships of Four Grouper Species in Genus Epinephelus Sampled from Sea Area of Southern China |
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| L&#; Feng-Hu,HAN Jie,DONG Ying and CAI Hou-Cai.Genetic Diversity and Phylogenetic Relationships of Four Grouper Species in Genus Epinephelus Sampled from Sea Area of Southern China[J].Chinese Journal of Zoology,2010,45(6):9-18. | |
| Authors: | L&#; Feng-Hu HAN Jie DONG Ying and CAI Hou-Cai |
| Institution: | Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering,College of Life Sciences,Beijing Normal University,Beijing 100875;Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering,College of Life Sciences,Beijing Normal University,Beijing 100875;Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering,College of Life Sciences,Beijing Normal University,Beijing 100875; Beijing Fisheries Research Institute,Beijing 100068,China;Nanji Islands National Marine Natural Reserve Administration,Wenzhou 325014 |
| Abstract: | Genetic diversity and phylogenetic relationships of four morphologically similar and sympatric grouper species,namely Yellow grouper(Epinephelus awoara),Hongkong grouper(E.akaara),Duskytail grouper(E.bleekeri) and Honeycomb grouper(E.merra) were investigated.Specimens were sampled from sea area of southern China.Calculation based on genotypes of nine microsatellite DNA loci showed that the genetic diversity of the four grouper species was high with the mean number of alleles,average polymorphic informative ... |
| Keywords: | Grouper Microsatellite mtDNA Cyt b Genetic diversity Molecular phylogeny |
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