匹拉米洞法、邻联甲苯胺法和联苯胺法 检测林麝便隐血的比较

圈养林麝(Moschusberezovskii)长期受困于消化道类疾病,尤其是肠道炎症性疾病患病率和死亡率一直居高不下。粪便检测是评估野生动物消化系统是否存在出血情况的有效方法之一,并且在圈养林麝肠道健康状况评估及肠道炎症性疾病临床诊断等方面提供了一定的诊断依据。粪便隐血在消化道出血诊断中有广泛的临床诊断价值。基于此,本研究用新鲜的林麝血液进行稀释,来探究匹拉米洞法、邻联甲苯胺法和联苯胺法三种方法对林麝血液浓度的灵敏度范围。检测结果显示,匹拉米洞法的最低敏感性检测浓度为0.05 mg/L,敏感性范围远大于邻联甲苯胺法(0.40 mg/L)和联苯胺法(100.00 mg/L)。分别利用三种检测方法对林麝粪便潜血进行检测,比较检测结果的阳性率,结果显示,匹拉米洞法的检测效果优于其他两种方法,阳性率分别为匹拉米洞法检测法10.13%、邻联甲苯胺法检测法2.56%和联苯胺法检测法0,差异有诊断学意义(P 0.05)。而且在操作上,匹拉米洞法更加简便快捷。故在诊断林麝消化道出血时,采用匹拉米洞法进行林麝便隐血的检测更加准确便捷。

Detection of Fecal Occult Blood in the Stool of Forest Musk Deer (Moschus berezovskii): Comparison among Pyramidon Chemical Method, O-toluidine Chemical Method and Benzidine Chemical Method

The high morbidity and mortality of captive Forest Musk Deer (Moschus berezovskii) due to the digestive tract diseases, especially the intestinal inflammatory diseases have been a major problem. Fecal detection is a basis for evaluating the health status of the digestive system of the wild animals and the clinical diagnosis of the intestinal inflammatory diseases. The fecal occult blood has a wide range of clinical diagnostic values in the diagnosis of gastrointestinal bleeding. In this study, diluted fresh Forest Musk Deer blood was utilized to explore the sensitivity ranges of three methods (pyramidon chemical method, o-toluidine chemical method and benzidine chemical method) for the determination of the blood concentration in the Forest Musk Deer feces. The results showed that the minimum sensitivity test concentration of the pyramidon method was 0.05 mg/L, more sensitive than that of o-toluidine method (0.4 mg/L) or benzidine method (100 mg/L). The positive rate of gastrointestinal bleeding revealed by the pyramidon method was significantly higher when compared to the other two methods, with the positive rate of pyramidon method 10.13%, o-toluidine chemical method of 2.56% and benzidine chemical method of 0 (P < 0.05). The operation of the pyramidon method was more convenient and rapid. Therefore, the detection of fecal occult blood in the Forest Musk Deer by pyramidon method is more accurate and convenient.