| 猪卵母细胞蛋白质组双向电泳体系的建立及初步分析 |
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| 引用本文： | 胡惠忠,付强,卢晟盛,张明,陆阳清,蒙冰,卢克焕.猪卵母细胞蛋白质组双向电泳体系的建立及初步分析[J].动物学杂志,2008,43(4):67-71. |
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| 作者姓名： | 胡惠忠 付强 卢晟盛 张明 陆阳清 蒙冰 卢克焕 |
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| 作者单位： | 广西亚热带生物资源保护利用重点实验室广西大学,南宁,530004 |
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| 基金项目： | 国家自然科学基金，广西科学基金 |
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| 摘 要： | 建立了猪(Sus scrofa)卵母细胞蛋白质双向电泳平台,并对裂解液的组成、样品处理、双向电泳程序等相关技术进行优化,得到清晰的微量卵母细胞蛋白质的电泳图谱.利用上述优化后的体系分别对未成熟和成熟的猪卵母细胞进行双向电泳分析,并用ImageMaser软件对图谱进行比对分析.结果表明,电泳图谱上大约有800个左右的蛋白点,其中差异蛋白35个,包括上调蛋白22个及下调蛋白13个.说明基于双向电泳的蛋白质组学可以用于卵母细胞成熟的蛋白表达差异的研究.
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| 关 键 词： | 猪 卵母细胞 双向电泳 蛋白组学 |
| 收稿时间： | 2007/10/28 0:00:00 |
| 修稿时间： | 2008/4/30 0:00:00 |
Two-dimensional Electrophoresis Analysis of Porcine Oocyte Proteome: a Preliminary Study |
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| HU Hui-Zhong,FU Qiang,LU Sheng-Sheng,ZHANG Ming,LU Yang-Qing,MENG Bing and LU Ke-Huan.Two-dimensional Electrophoresis Analysis of Porcine Oocyte Proteome: a Preliminary Study[J].Chinese Journal of Zoology,2008,43(4):67-71. |
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| Authors: | HU Hui-Zhong FU Qiang LU Sheng-Sheng ZHANG Ming LU Yang-Qing MENG Bing and LU Ke-Huan |
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| Institution: | HU Hui-Zhong FU Qiang LU Sheng-Sheng ZHANG Ming LU Yang-QingMENG Bing LU Ke-Huan(Guangxi Key Laboratory of Subtropical Bio-Resource Conservation , Utilization,Guangxi University,Nanning 530004,China) |
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| Abstract: | The two-dimensional electrophoresis(2-DE) technique for Porcine(Sus scrofa) oocyte proteome analysis was established.The components of protein lysis solution,sample treatment,and 2-DE procedure were optimized.A profile of proteins extracted from Porcine oocytes was obtained clearly.The protein expression profiles of immature and in vitro matured Porcine oocytes were compared. The ImageMaster 2-DE software was used to characterize different proteins.About 800 protein spots were identified,of which 22 protein... |
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| Keywords: | Porcine Oocyte Two-dimensional electrophoresis Proteomics |
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