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小麂、赤麂、黑麂mtDNA序列变异性及反映的进化关系
引用本文:单祥年,施燕峰,张海军,徐春宏,李健,郑爱玲.小麂、赤麂、黑麂mtDNA序列变异性及反映的进化关系[J].动物学杂志,2004,39(4):35-39.
作者姓名:单祥年  施燕峰  张海军  徐春宏  李健  郑爱玲
作者单位:东南大学基础医学院遗传中心,南京,210009;南京师范大学生命科学院,南京,210097;东南大学基础医学院遗传中心,南京,210009
基金项目:国家自然科学基金资助项目 (No .39970 1 1 )
摘    要:利用已测定的鹿科麂亚科动物小麂、赤麂、黑麂的线粒体全基因组序列,统计它们各自连接在一起的13个蛋白编码基因、22个tRNA基因、2个rRNA基因和1个控制区序列的碱基长度和组成,计算rRNA基因遗传距离,估算分歧时间,比较蛋白编码基因的碱基水平和氨基酸水平上的差异,基于连接在一起的13个氨基酸序列,以羊为外群,通过邻位相连法和最大简约性法构建进化树,探讨小麂、赤麂、黑麂的进化关系。结果表明,小麂是较原始的物种,赤麂和黑麂较为近缘,是从类似小麂的祖先演化而来。

关 键 词:麂亚科  线粒体基因组  进化关系
文章编号:0250-3263(2004)04-35-05

mtDNA Sequences Variability of Munliacus reevesi,M.muntjak and M.crinifrons as well as the Related Phylogenetic Relationship
Abstract:Using the mitochondrial genomes of the Muntiacus reevesi,M.muntjak and M.crinifrons sequenced by our lab,we calculated the sequence lengths and base compositions of their individual concatenated sequences of 13 protein coding genes,22 tRNA genes,2 rRNA genes and their control regions.The genetic distances based on the rRNA genes were calculated and their divergence time was estimated.The differences between the protein coding genes and between the amino acid sequences were also compared.The phylogenetic tree was constructed with Neighbor Joining method and Maximum Parsimony method based on the concatenated amino acid sequences and the mtDNA sequence of O.aries in order to discuss their phylogenetic relationships.The results show that M.reevesi is more antique than M.muntjak and M.crinifrons.M.muntjak and M.crinifrons were diverged from an ancestor analogized with M.reevesi.
Keywords:Muntiacinae  Mitochondrial genome  Phylogenetic relationship
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