| Na+、K+、Mg2+、Ca2+和葡萄糖溶液作为授精-激活介质对中华鲟精子受精率的影响 |
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| 引用本文： | 甘芳,杜浩,危起伟,陈细华,刘鉴毅,郑跃平.Na+、K+、Mg2+、Ca2+和葡萄糖溶液作为授精-激活介质对中华鲟精子受精率的影响[J].动物学杂志,2009,44(1):106-111. |
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| 作者姓名： | 甘芳 杜浩 危起伟 陈细华 刘鉴毅 郑跃平 |
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| 作者单位： | 1. 农业部淡水生物多样性保护与利用重点开放实验室,华中农业大学水产学院,武汉,430070;中国水产科学研究院长江水产研究所,荆州,434000;中国水产科学研究院淡水渔业研究中心,无锡,214081
2. 中国水产科学研究院长江水产研究所,荆州,434000;中国水产科学研究院淡水渔业研究中心,无锡,214081 |
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| 基金项目： | 国务院三峡办重点项目，国家自然科学基金重点项目，湖北省科技攻关项目 |
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| 摘 要： | 以不同浓度的NaCl、KCl、MgCl2、CaCl2溶液和葡萄糖溶液作为授精介质,研究了中华鲟(Acipenser sinensis)的受精效果.结果显示,适量的阳离子和葡萄糖作为激活授精介质时中华鲟卵受精率都有所提高.在实验设置浓度范围内25 mmol/L NaCI溶液、0.1 mmol/L KCl溶液、1 mmol/L MgCl2溶液、1 mmol/LCaCh溶液和50 mmol/L葡萄糖溶液浓度下,受精率分别可达到最高值,依次为87.72%、86.82%、82.24%、89.76%、80.92%.随着实验浓度继续增加,受精率反而呈下降趋势.结果显示,作为人工配制的中华鲟精子授精一激活介质,最适NaCI溶液浓度在25 mmol/L附近,最适葡萄糖溶液浓度在25 mmol/L附近,最适KCI溶液浓度≤0.1 mmol/L,最适MgCl2溶液浓度≤1 mol/L,最适CaCh溶液浓度≤1 mmol/L.
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| 关 键 词： | 中华鲟 精子活力 受精率 |
| 收稿时间： | 2008/6/27 0:00:00 |
| 修稿时间： | 2008/11/18 0:00:00 |
Effect of Na+,K+,Mg2+),Ca2+ and Glucose Solutions as Insemination-activation Media on the Fertilization of Acipenser sinensis Milt |
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| GAN Fang,DU Hao,WEI Qi-Wei,CHEN Xi-Hu,LIU Jian-Yi and ZHENG Yue-Ping.Effect of Na+,K+,Mg2+),Ca2+ and Glucose Solutions as Insemination-activation Media on the Fertilization of Acipenser sinensis Milt[J].Chinese Journal of Zoology,2009,44(1):106-111. |
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| Authors: | GAN Fang DU Hao WEI Qi-Wei CHEN Xi-Hu LIU Jian-Yi and ZHENG Yue-Ping |
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| Institution: | Key Laboratory of Freshwater Biodiversity Conservation and Utilization,Ministry of Agriculture of China,College of Fisheries,Huazhong Agricultural University,Wuhan 430070; Yangtze River Fisheries Research Institute,Chinese Academy of Fisheries Science,Jingzhou 434000; Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081,China;angtze River Fisheries Research Institute,Chinese Academy of Fisheries Science,Jingzhou 434000; Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081,China;Key Laboratory of Freshwater Biodiversity Conservation and Utilization,Ministry of Agriculture of China,College of Fisheries,Huazhong Agricultural University,Wuhan 430070; Yangtze River Fisheries Research Institute,Chinese Academy of Fisheries Science,Jingzhou 434000; Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081,China;Yangtze River Fisheries Research Institute,Chinese Academy of Fisheries Science,Jingzhou 434000; Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081,China;Yangtze River Fisheries Research Institute,Chinese Academy of Fisheries Science,Jingzhou 434000; Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081,China;Key Laboratory of Freshwater Biodiversity Conservation and Utilization,Ministry of Agriculture of China,College of Fisheries,Huazhong Agricultural University,Wuhan 430070; Yangtze River Fisheries Research Institute,Chinese Academy of Fisheries Science,Jingzhou 434000; Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081,China |
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| Abstract: | The fertilization of Chinese Sturgeon (Acipenser sinensis) milt was assessed by using different concentrations of NaCl,KCl,MgCl_2,CaCl_2 or glucose solution as the insemination-activation medium.The results showed that the fertilization rates of Chinese Sturgeon were all improved when suitable concentrations of NaCl,KCl,MgCl_2,CaCl_2 and glucose solutions were used,and that the fertilization rates reached the highest when the following concentrations of solutions were used: 87.72% in 25 mmol/L NaCl,86.82% i... |
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| Keywords: | Chinese Sturgeon (Acipenser sinensis) Sperm motility Fertilization rate |
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