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细胞松弛素B对鲍受精卵超微结构的影响
引用本文:孙振兴,王如才,姜明,刘晓云.细胞松弛素B对鲍受精卵超微结构的影响[J].动物学杂志,2002,37(5):14-17.
作者姓名:孙振兴  王如才  姜明  刘晓云
作者单位:1. 烟台师范学院生物学系,烟台,264025
2. 青岛海洋大学,青岛,266003
基金项目:国家高技术研究发展计划(863计划),863819-0101,
摘    要:细胞松弛素B(CB)作为一种微丝解聚剂,可用于诱导贝类三倍体,但由于其毒副作用导致了受精卵死亡和发育异常。通过透射电镜观察比较了皱纹盘鲍(Haliotis discus hannai)正常受精卵与CB处理后的受精卵亚显微结构的变化,结果显示,CB对线粒体等细胞器的破坏,造成了受精卵的代谢缺陷,是导致鲍受精卵孵化率低的原因之一。

关 键 词:细胞松弛素B  鲍受精卵  超微结构  皱纹盘鲍
文章编号:0250-3263(2002)05-14-04

Effect of Cytochalasin B on the Ultrastructure of Fertilized Eggs of Abalone (Haliotis discus hannai)
Abstract:Cytochalasin B(CB) is an inhibitor of microfilament. It is extensively used for polyploid induction in shellfish. However because of its toxicity CB treatment often results death and abnormal development of the fertilized eggs. We have observed the ultrastructure of normal fertilized eggs and CB treated eggs of the abalone( Haliotis discus hannai ), and compared normal and abnormal eggs with transmission electron microscope (TEM). The results indicate that CB breaks down cell organs such as mitochondria etc.; it causes the metabolic disturbance of fertilized eggs. This is one of the reasons how the low hatch rate of abalone fertilized eggs.
Keywords:Cytochalasin B  Abalone(  Haliotis discus hannai  )  Fertilized eggs  Ultrastructure
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