| 基于ISSR标记和线粒体Cyt b基因分析高原鼠兔的遗传多样性及其遗传分化 |
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| 引用本文： | 葛艳丽,林恭华,慈海鑫,张同作,唐利洲,苏建平.基于ISSR标记和线粒体Cyt b基因分析高原鼠兔的遗传多样性及其遗传分化[J].动物学杂志,2009,44(4):34-40. |
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| 作者姓名： | 葛艳丽 林恭华 慈海鑫 张同作 唐利洲 苏建平 |
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| 作者单位： | 1. 中国科学院高原生物适应与进化重点实验室中国科学院西北高原生物研究所,西宁,810001;中国科学院研究生院,北京,100049
2. 中国科学院高原生物适应与进化重点实验室中国科学院西北高原生物研究所,西宁,810001 |
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| 基金项目： | 中国科学院知识创新工程领域前沿项目(No.CXLY-2002-3) |
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| 摘 要： | 高原鼠兔(Ochotona curzoniae)是青藏高原的特有动物和关键种。本实验应用ISSR分子标记和细胞色素b(Cytochrome b,Cytb)基因序列分析了雅鲁藏布江两岸高原鼠兔4个种群的遗传多样性和遗传分化。结果显示,两种标记所得到的4个种群的遗传多样性都较高,并以Cytb基因为指标的遗传多样性水平在种群间体现出较大差异。在ISSR标记中,分子方差分析(AMOVA)表明种群间的遗传变异为13.50%,种群分化较低且UPGMA聚类时江北岸与南岸的种群有交叉;而在Cytb基因中遗传变异主要发生在种群间,占79.24%,种群间存在着显著的遗传分化,且江北岸和南岸的两个种群分别聚为一类。研究结果表明,mtDNA基因在反映种群遗传多样性和遗传分化上较ISSR分子标记相对具有优势。
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| 关 键 词： | 高原鼠兔 ISSR 细胞色素b基因 遗传多样性 遗传分化 |
| 收稿时间： | 2008/12/22 0:00:00 |
| 修稿时间： | 2009/5/18 0:00:00 |
Genetic Diversity and Differentiation of Ochotona curzoniae Based on ISSR and Cyt b Gene |
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| GE Yan-Li,LIN Gong-Hu,CI Hai-Xin,ZHANG Tong-Zuo,TANG Li-Zhou and SU Jian-Ping.Genetic Diversity and Differentiation of Ochotona curzoniae Based on ISSR and Cyt b Gene[J].Chinese Journal of Zoology,2009,44(4):34-40. |
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| Authors: | GE Yan-Li LIN Gong-Hu CI Hai-Xin ZHANG Tong-Zuo TANG Li-Zhou and SU Jian-Ping |
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| Institution: | GE Yan-Li LIN Gong-Hua CI Hai-Xin ZHANG Tong-Zuo TANG Li-Zhou SU Jian-Ping ( Key Laboratory of Adaptation , Evolution of Plateau Biota,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810001, Graduate School of Chinese Academy of Sciences,Beijing 100049,China) |
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| Abstract: | The Plateau Pika(Ochotona curzoniae) is endemic to the Qinghai-Tibetan Plateau and is a key species in the alpine meadow ecosystem.In this paper,genetic diversity and differentiation of four populations of Plateau Pika from both banks of the Brahmaputra River were assessed with inter-simple sequence repeat(ISSR) and Cyt b gene sequences.Our results showed that the genetic diversity of Plateau Pika was relatively high and there were much larger deviations among populations based on Cyt b gene than those base... |
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| Keywords: | ISSR |
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