高氟对3月龄公鸡生殖细胞的损伤作用

为了探讨高氟对鸡生殖细胞损伤作用,选用3月龄蛋公鸡10只,实验组和对照组各一组,每组5只,实验组在饮水中添加3.25 g/L的氟化钠,对照组饮用自来水,实验周期为120 d。分别于60 d、80 d、100d、120 d采集精液,检测精子密度、早期精细胞微核率、精子畸形率及精子活率。120 d后,处死动物,取两侧睾丸,称重并测量大小。结果表明,氟化钠可致精子数量减少,活率降低,精子畸形率和微核率上升,并呈明显的时间-效应关系。比较两组睾丸重量和大小,差异极显著(P<0.01)。说明高氟对鸡生殖细胞有遗传损伤作用,高氟可能是致突变物。

Germ Cell Damage Caused by Excessive Fluoride in Three-month Old Cocks

To study the effects of excessive fluorine on germ cells,10 three-month old cocks were randomly divided into 2 groups.The cocks in experimental group were fed with water containing 3.25 g/L sodium fluoride and the control individuals were fed with drinking running water for 120 days.Semen was collected after 60,80,100,and 120 days respectively,and sperm density,micronucleus rate,sperm deformity rate and of active sperm rate were counted.Animals were killed after 120 days,and testes were collected and weighed.The results showed that sperm density and active sperm rate were lower than those of control group,while sperm deformity rate and micronucleus rate were higher than the control.These parameters showed obvious correlativity with time.Size and weight of testes were compared,and the difference was significant(P<0.01).These results show that excessive fluorine damages the germ cells of cocks,and fluorine may be mutagenic.