三种不同科属蛙类外周血细胞的比较

采用快速瑞氏-姬姆萨(Wright’s-Giemsa’s)染色法对斑腿泛树蛙(Polypedates megacephalus)和沼水蛙(Hylarana guentheri)的外周血细胞进行观察,并与北部湾棱皮树蛙(Theloderma corticale)已有的数据进行了比较。结果显示,三种蛙的血细胞均由红细胞、白细胞和血栓细胞组成,三种蛙的血细胞在形态结构上具有一定的共性,但在形态大小、数量上差异显著。沼水蛙的红细胞长径和短径均显著大于其余两种,分别为(23.56±2.78)μm和(14.25±1.62)μm;3种蛙的嗜中性粒细胞和小淋巴细胞在大小上没有明显差别,但北部湾棱皮树蛙的嗜酸性粒细胞和嗜碱性粒细胞的长径、短径均显著大于其他两种蛙。北部湾棱皮树蛙的红细胞数量显著少于其余两者,为(24.33±9.46)×10⁴/mm³;斑腿泛树蛙白细胞数量显著少于其余两种蛙,为(1.40±0.40)×10⁴/mm³。这些差异的产生可能与其生活环境以及生活习性有关。

The Comparison of Peripheral Blood Cells in Three Frog Species from Different Families and Genera

Peripheral blood cells were characterized in Polypedates megacephalus and Hylarana guentheri by the Wright-Giemsa staining method,and the data were compared with those previously recorded in Theloderma corticale.The results showed that the peripheral hematocytes of all three species were composed of erythrocytes,leukocytes and thrombocytes.The blood cells of the three species had the same commonality in morphology and structure(Fig.1),while there were also remarkable differences in the shape,size and quantity(Fig.2,Table 1-4).The nuclei of the red blood cells of H.guentheri were(23.56±2.78)μm×(14.25±1.62)μm in long diameter and short diameter,respectively,significantly larger than in P.megacephalus and T.corticale.There was no significant difference in size between neutrophils and lymphocytes,but the long diameter and short diameter of neutrophils and lymphocytes of T.corticale were the largest.In terms of the number of cells,the number of red blood cells in T.corticale was(24.33±9.46)×10⁴/mm³,significantly lower than that in P.megacephalus or H.guentheri.while the number of white blood cells of P.megacephalus was(1.40±0.40)×10⁴/mm³,which was the least among the three species,significantly different from the other two species(P<0.05).These differences may be related to living environment and living habits of the frogs.