大熊猫精液超低温冷冻的比较

对卧龙自然保护区大熊猫研究中心的9只雄性大熊猫电刺激采精，比较研究了稀释液中甘油含量、精液离心、不同稀释液和冷冻方法对大熊猫精液超低温冷冻保存后的活力、运动状态和顶体的影响。稀释液中甘油的含量为4％－5％较好，冻精解冻后的活力和顶体的正常率能保持鲜精的一半。离心和未离心的精液经超低温冷冻，解冻后的活力和运动状态都较接近。TEST和SFS两种稀释液的效果没有明显的差异。细管的冷冻过程较颗粒方便、快捷，时间容易控制，是一种较好的超低温冷冻精液的方法。

Study for Freezing Semen of Giant Pandas Study for Freezing Semen of Giant Pandas Study for Freezing Semen of Giant Pandas

Nine males at the Research and Conservation Center for Giant Panda in Wolong Nature Reserve were used for this study. Semen was collected by electroejaculation. Influence of glycerol contents, sperm washing, cryodiluents, freezing methods on sperm motility,status and acrosome were studied. Cryodiluent with 4%～5% glycerol was effective for freezing giant panda sperm. Washing sperm (to remove seminal fluid) might improve post-thaw motility and forward progressive status. SFS and TEST cryodiluent showed same effectiveness in freezing giant panda semen. To freeze in straws was easier and faster than to freeze in pellets.