细胞色素c在盘基网柄菌细胞凋亡中的作用

细胞色素c在细胞凋亡中发挥着重要的作用,其作用机理在高等真核生物及低等真核生物酵母中已经比较清楚,但在盘基网柄菌(Dictyostelium discoideum)中的作用却没有相关报道.所以我们用western blot和实时荧光定量PCR的方法分别测定了盘基网柄菌前柄细胞和前孢子细胞中细胞色素c的含量及表达量的变化...

Role of Cytochrome c in Apoptosis of Dictyostelium discoideum

Cytochrome c plays an important role in apoptosis of eukaryotes and yeast, but there are few reports about its role in Dictyostelium discoideum. Western blot and S Fluorescence Quantitative PCR were used to detect the quantity and expression changes of cytochrome c in prestalk cells and prespore cells, in order to reveal the role of cytochrome c in apoptosis of dityostelium prestalk cells. In process of 16-20 h development of apoptotic prestalk cells, the amount of mitochondrial cytochrome c showed a decreasing tendency while the amount of cytoplasmic cytochrome c showed an increasing tendency inversely. Total amount of cytochrome c in prestalk cells was higher than that in prespore cells. Cytochrome c gene expression in prestalk cells was higher than that in prespore cells, and showed an increasing tendency, but cytochrome c gene expression in prespore cell was relatively stable. These results indicate that cytochrome c, to some extent, participates in the apoptosis of Dictyostelium prestalk cells.