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濒危平胸龟两个自然居群的ISSR分析
引用本文:马丽莎,郑光明,朱新平,刘毅辉,陈永乐,罗建仁.濒危平胸龟两个自然居群的ISSR分析[J].动物学杂志,2007,42(6):13-20.
作者姓名:马丽莎  郑光明  朱新平  刘毅辉  陈永乐  罗建仁
作者单位:1. 中国水产科学研究院珠江水产研究所,广州,510380;广东海洋大学,湛江,524025
2. 中国水产科学研究院珠江水产研究所,广州,510380
基金项目:广东省自然科学基金(No04001499)资助
摘    要:利用22个ISSR引物研究了28个来自广东从化居群和21个来自福建寿宁居群的野生平胸龟(Platysternon megacephalum)的遗传多样性。结果表明,分布于这两个地区的平胸龟总居群和居群内均具有较高的遗传多样性,而广东从化居群遗传多样性高于福建寿宁居群。居群间的遗传变异度FST=0.3715(P<0.001),遗传分化系数Gst为0.2059。NJ树聚类分析显示,广东从化居群和福建寿宁居群各自聚成两个类群,有较明显的居群分化,具有一定的地理区域性,但基因流Nm为1.9279,这表明平胸龟两自然居群间存在一定基因流。此外,结合BSA技术,从11条引物中筛选出11个可作为鉴别广东从化居群或福建寿宁居群的分子标记。

关 键 词:平胸龟  自然居群  遗传差异  ISSR  BSA
文章编号:0250-3263(2007)06-13-08
收稿时间:2007/4/23 0:00:00
修稿时间:2007年4月23日

Genetic Diversity in Two Natural Populations of Platysternon megacephalum as Revealed by ISSR Technique
MA Li-Sh,ZHENG Guang-Ming,ZHU Xin-Ping,LIU Yi-Hui,CHEN Yong-Le and LUO Jian-Ren.Genetic Diversity in Two Natural Populations of Platysternon megacephalum as Revealed by ISSR Technique[J].Chinese Journal of Zoology,2007,42(6):13-20.
Authors:MA Li-Sh  ZHENG Guang-Ming  ZHU Xin-Ping  LIU Yi-Hui  CHEN Yong-Le and LUO Jian-Ren
Institution:Pearl River Fisheries Research Institute,Chinese Academy Fishery of Sciences,Guangzhou 510380; Guangdong Ocean University,Zhanjiang 524025,China;Pearl River Fisheries Research Institute,Chinese Academy Fishery of Sciences,Guangzhou 510380;Pearl River Fisheries Research Institute,Chinese Academy Fishery of Sciences,Guangzhou 510380;Pearl River Fisheries Research Institute,Chinese Academy Fishery of Sciences,Guangzhou 510380;Pearl River Fisheries Research Institute,Chinese Academy Fishery of Sciences,Guangzhou 510380;Pearl River Fisheries Research Institute,Chinese Academy Fishery of Sciences,Guangzhou 510380
Abstract:Genetic diversity of endangered Big-headed Turtle,28 individuals from Conghua,Guangdong and 21 individuals from Shouning,Fujian,was determined by using ISSR technique.There was high genetic diversity between the two populations and within the same population.The genetic diversity of Conghua population was higher than that of Shouning population.Genetic differentiation mainly occurred between populations(F-ST =0.371 5,G-st =0.205 9),but there was also gene flow between natural populations(N-m=1.927 9).UPGMA cluster analysis showed that the 49 individuals were divided into two clusters corresponding to the two populations.In addition,11 specific ISSR markers which can distinguish Conghua population from Shouning population were selected from 11 primers in two populations,using ISSR and Bulked Segregant Analysis(BSA).
Keywords:Platysternon megacephalum  Nature population  Genetic diversity  ISSR analysis  BSA
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