两种蛇Sox基因的PCR-SSCP分析 * |
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引用本文: | 杨,超,杨传秀,聂刘旺.两种蛇Sox基因的PCR-SSCP分析 *[J].动物学杂志,2003,38(1):8-12. |
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作者姓名: | 杨 超 杨传秀 聂刘旺 |
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作者单位: | 1. 安徽师范大学生命科学学院,芜湖,241000 2. 皖南医学院生理学教研室,芜湖,241001 |
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基金项目: | 安徽省自然科学基金,安徽省校青年基金,01043202,125032,, |
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摘 要: | 采用PCR技术,以特异扩增人SRY基因HMG-box保守区的一对引物,扩增了乌梢蛇和赤链蛇的Sox基因。结果 两种蛇雌雄个体与人一样,均能扩增出大小为220bp左右的基因片段。对扩增产物进行的SSCP分析发现两种蛇之间以及种内雌雄个体间单链迁移率略有差异,而与人有较大差异。本文为研究蛇类的性别决定机制及Sox基因的演化提供了分子生物学资料。
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关 键 词: | 蛇 Sox基因 PCR—SSCP分析 乌梢蛇 赤链蛇 性别决定机制 |
文章编号: | 0250-3263(2003)01-08-05 |
PCR Amplification and SSCP Analysis of the Sox Gene in Snakes Zaocys dhumnades and Dinodon rufozonatum |
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Abstract: | PCR was used to amplify the Sox gene of Zaocys dhumnades and Dinodon rufozonatum with a pair of primers, which can also amplify the conservative sequence of the human SRY gene. The results suggest that both male and female snakes have the Sox gene, which has a length about 220?bp, equal to that of human SRY gene. The result of SSCP analysis showed that there were many differences in the Sox gene sequence between snakes and humans, but few difference between males and females of the same species or between the two species of snakes. These results are relevant to the study of sex\|determination in snakes and the evolution of the Sox gene. |
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Keywords: | Sox gene PCR\|SSCP Snake |
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