雌二醇对蒙古绵羊输卵管上皮细胞内β-防御素(sBD-1)表达的影响

为了探索雌二醇与β-防御素(sBD-1)表达量的关系,体外模拟蒙古绵羊(Ovis aries)生理周期.用添加雌二醇浓度10-11、10-10、10-9、10-8、10-7、10-6 mol/L的培养液及不添加雌二醇的培养液(即对照组)分别培养蒙古绵羊输卵管上皮细胞,作用24 h、48 h、72 h后,提取细胞总RNA,应用SYBR Green I荧光定量RT-PCR法进行定量分析.结果显示.添加不同浓度雌二醇培养的输卵管上皮细胞中sBD-1的相对表达量0.000 740～0.001 758,与对照组0.000 190之间差异显著(P＜0.05),且小于10-8 mol/L雌二醇添加浓度与sBD-1基因相对表达量变化基本呈正相关.此结果为进一步研究雌激素参与机体防御功能奠定了基础.

Effects of Estradiol on the Expression of β-Defensin-1(sBD-1)in Epithelial Cells of Fallopian Tubes in the Mongolian Sheep

Fallopian tube epithelial cells of the Mongolian Sheep(Ovis aries) were cultured in the medium containing estradiol.Concentration of estradiol was 10~(-11),10~(-10),10~(-9),10~(-8),10~(-7),10~(-6) or 0 mol/L.After culturing for 24,48,and 72 hours,total RNA was extracted and the cDNA encoding sBD-1 was amplified by RT-PCR,respectively.The results show that sBD-1 expression in Fallopian tube epithelial cells is evidently affected by estradiol treatment.There is a positive correlation between sBD-1 expression ...