粤东海域口虾蛄遗传多样性

采用线粒体COI基因序列对粤东汕尾和深圳2个海域口虾蛄(Oratosquilla oratoria)的遗传多样性进行了分析.研究表明,所分析的口虾蛄mtDNA CO I基因(592 by)共检测到21个变异位点,占总位点的3.55%.转换和颠换位点数分别为18和3个,碱基替换的饱和性分析表明,口虾蛄CO I基因碱基替换...

Genetic Diversity Analysis of Mantis Shrimps (Oratosquilla oratoria) from the Eastern Coast of Guangdong Province

As a routine and important polymorphic marker,mitochondrial cytochrome oxidase subunit Ⅰ(CO Ⅰ) gene was used to analyze the genetic diversity for two populations of Oratosquilla oratoria from Shenzhen and Shanwei coast of Guangdong Province.The result revealed that 21 of the 592 bp(3.55%) nucleotides were variable,among which 18 and 3 sites were identified as transition and transversion respectively.The substitution saturation analysis indicated that COⅠ gene was not satured in O.oratoria and could be used as an effective molecular tool for population genetic studies.Totally,15 haplotypes were detected.The nucleotide diversity and haplotype diversity were 0.007 55 and 0.971 respectively.The fixation index(FST) was 0.036 between two populations,suggesting little differentiation between them.There was no geographic clustering of haplotypes in the UPGAM tree and the network.Genetic distances among haplotypes ranged from 0.047 6 to 0.428 6,with an average value of 0.214 1.In summary,a high level of genetic diversity and little genetic differentiation are observed in O.oratoria from the eastern coast of Guangdong province.