小地老虎UV视蛋白基因的克隆及序列分析

视蛋白是感光物质的重要组成成分,是动物感知周围光环境的重要途径之一。本文以小地老虎(Agrotis ypsilon)3日龄成虫为材料,利用RT-PCR和RACE末端扩增技术克隆得到小地老虎UV视蛋白基因的cDNA序列。序列分析表明,小地老虎视蛋白基因的cDNA序列1 632 bp,包括一个1 140 bp的完整开放阅读框架,编码379个氨基酸,理论蛋白分子量(Mw)41.50 ku,等电点(pI)7.56。GenBank登录号为JN185654。UV视蛋白包括7个跨膜拓扑结构和一个G蛋白偶联受体家族,第107位赖氨酸与UV视蛋白的紫外敏感性有重要关系。同源性分析显示,小地老虎UV视蛋白基因与其他昆虫的UV视蛋白基因具有较高同源性。本研究对深入探究UV视蛋白在动物夜行生活中的作用具有重要意义。

Molecular Cloning and Sequence Analysis of UV Opsin in Agrotis ypsilon

Opsin is the main content of photoactive substances,and it is important for the light perception of animals.Total RNA was isolated from the third day adult of the Agrotis ypsilon.The cDNA sequence was cloned by RT-PCR and rapid amplification of cDNA ends (RACE).UV opsin cDNA sequence is 1 632 bp in length,containing an open reading frame of 1 140 bp,which encodes a polypeptide of 379 amino acids residues.The predicted molecular weight of the amino acid is about 41.50 ku,with an isoelectric point of 7.56.GenBank accession number: JN185654.The analysis of phylogenetic relationship showed that the cloned gene had high amino acid sequences homology with other insect UV opsins.The molecular cloning of UV opsin gene may play an important role in the study of nocturnal insect vision in the night.