角类肥蛛体躯不同部位酯酶同工酶的比较分析

运用聚丙烯酰胺凝胶电泳的方法对角类肥蛛(Lariniodes cornuta)头胸部和腹部的酯酶同工酶酶谱进行了比较分析。结果表明,角类肥蛛的酯酶是单体酶,头胸部和腹部的酯酶酶谱差异显著。腹部的酯酶呈现4个位点:Est-1、Est-2、Est-3、Est-4。Est-1和Est-4位点为纯合基因型,Est-2和Est-3位点为杂合基因型。头胸部的酯酶仅表现出2个位点:Est-2和Est-3,且这2个位点是纯合基因型。不同个体之间头胸部的酯酶没有明显差异,Est-2b和Est-3a可以作为鉴别角类肥蛛的特征酶带;腹部的酯酶则存在明显的个体差异,在Est-2和Est-3位点的基因杂合度为h2=h3=0·4779。由此可见,酯酶同工酶可以作为角类肥蛛遗传变异的分子标记,是研究个体间遗传差异、居群的遗传结构以及种间进化关系的基础。

Difierences in Esterase Isozymes in Difierent Parts of Lariniodes cornuta

The genetic structure of esterase colorimetric(EST) in cephalothorax and abdomen of Lariniodes cornuta was studied by using isozyme analysis with polyacryamide gel electrophoresis(PAGE).As a result,EST in L.cornuta was a monomeric enzyme and there were some important differences between cephalothorax and abdomen in the EST zymogram.EST in abdomen presented Est-1,Est-2,Est-3,Est-4 loci.Est-1 and Est-4 were homozygote while the others(Est-2 and Est-3) were heterozygote.However,EST in cephalothorax only stated two loci:Est-2 and Est-3.Both of them were homozygote.Furthermore,the EST zymogram of cephalothorax was alike in different individuals.And Est-2b and Est-3a could be used as the characteristic zymogram for differentiating L.cornuta.Nevertheless,some differences appeared in the abdomen zymogram of different individuals.The frequency of heterozygote(h_i) for the Est-2 and Est-3 was 0.477 9.Besides we also discussed the significance of our findings for further studies on analyzing genetic variation in individuals and in populations and phylogenetic analysis.