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不同固定剂保存动物组织标本对RAPD反应的影响
引用本文:王义权,周开亚,徐珞珊,徐国均.不同固定剂保存动物组织标本对RAPD反应的影响[J].动物学杂志,1999,34(1):33-37.
作者姓名:王义权  周开亚  徐珞珊  徐国均
作者单位:1. 南京师范大学生物系,南京,210097
2. 南京师范大学生物多样性与分子进化研究室,南京210097
3. 中国药科大学生药研究室,南京,210009
基金项目:中国科学院资助项目;39470098;
摘    要:为解决野外采集动物标本时,有效地保存好标本,并方便地带回实验室用于RAPD分析的难题。该研究以同一个体的冻存组织为对照,比较了从4种不同固定剂保存的组织标本中提取DNA,并用于RAPD扩增。

关 键 词:固定剂  标本保存  DNA提取

THE ANALYSES OF RAPD REACTION AFFECTED BY THE DNA TEMPLATE EXTRACTED FROM SAMPLES FIXED IN DIFFERENT FIXATIVES
WANG Yi-Quan,ZHOU Kai-Ya,XU Luo-Shan,XU Guo-Jun.THE ANALYSES OF RAPD REACTION AFFECTED BY THE DNA TEMPLATE EXTRACTED FROM SAMPLES FIXED IN DIFFERENT FIXATIVES[J].Chinese Journal of Zoology,1999,34(1):33-37.
Authors:WANG Yi-Quan  ZHOU Kai-Ya  XU Luo-Shan  XU Guo-Jun
Abstract:It is difficult to keep biological samples collected from the field fresh and transport safely to laboratory for RAPD analyses. In this study, the DNA templates extracted from muscles kept in different fixatives for more than 70 days in summer were compared in RAPD analyses and the DNA extracted from frozen muscles of same individuals was used as control. The result suggested that the muscle kept in 70% ethanol with 50mmol/L EDTA was quite good for template DNA extraction and RAPD reaction. At least, the sample could be in the fixative for two months at room temperature in summer without affection for DNA extraction and amplification.
Keywords:RAPD
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