大鼠睾丸生精小管上皮精子发生周期的PAS法判定

精子发生是一个包含生殖细胞成熟分裂的连续、复杂的动态过程，不同的生精小管，或同一生精小管不同区段的生精细胞的组合、分布均不相同。本文应用PAS染色法观察了大鼠睾丸生精小管上皮中各级生精细胞在精子发生过程的形态学变化特点。参照Clermont及Russell等制定的生精上皮时相的判定标准，根据生精上皮在精子发生过程中的各级生精细胞组合分布特点，把生精上皮分为ⅩⅣ个期。通过观察精子发生过程中生精上皮细胞组合的周期性形态变化特点，对精子发生过程进行精确划分，把精子发生这一连续、复杂的动态过程静止化，具体化，可以更加准确地描述和比较不同影响因素对生精小管上皮中各级生精细胞的组织学、病理学、毒理学变化。

Evaluation of Seminiferous Epithelium Stage in Rat Testis by PAS Staining

The purpose of this stuty is to evaluate the stage of seminiferous epithelium in adult rat testis.The cellular association was defined by the developmental stage of the spermatids,which themselves were characterized by their staining with the PAS (Periodic Acid Schiff) technique.According to the criterion of Clermont and Russell,the developmental stages can be easily identified by this technique.A cycle of the seminiferous epithelium could be divided into 14 periods,each of these periods was found to have a well defined cell association.By using this technique,researchers can learn the more precise features in rat spermatogenesis.