姜黄素对双酚A致小鼠卵巢氧化损伤的保护

本文旨在研究姜黄素(CRC)对双酚A(BPA)诱导的小鼠卵巢氧化损伤的保护作用。将28日龄雌性小鼠分为对照组、姜黄素组、双酚A组和双酚A加姜黄素组,连续灌胃6周。收集卵巢,通过活性氧(ROS)水平的检测、卵巢闭锁卵泡的观察以及3种关键抗氧化酶表达和活性的测定,研究姜黄素对双酚A诱发的卵巢氧化损伤的保护作用及机制。结果显示,与对照组相比,双酚A暴露后明显增加了卵巢的活性氧水平,造成氧化应激,提高了卵巢中有腔卵泡闭锁比例。与双酚A组相比,双酚A和姜黄素共同处理组降低了卵巢的活性氧水平和卵巢中有腔卵泡闭锁比例。双酚A暴露降低了卵巢超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPx)以及过氧化氢酶(CAT)的表达和活性,姜黄素逆转了双酚A诱导的3种抗氧化酶表达和活性的下降。结果表明,姜黄素可逆转双酚A通过氧化应激造成的卵巢损伤。

Protective Effect of Curcumin against Ovarian Oxidative Damage Induced by Bisphenol A in Mice

This work is aimed to investigate the protective effect of curcumin (CRC) against ovarian oxidative stress induced by bisphenol A (BPA) in mice. Firstly, in order to explore the appropriate protective concentration of curcumin, 28-day-old female mice were divided into six groups: control group, curcumin (200 mg/kg) group, bisphenol A (10 mg/kg) group, bisphenol A (10 mg/kg) + curcumin (50 mg/kg) group, bisphenol A (10 mg/kg) + curcumin (100 mg/kg) group, and bisphenol A (10 mg/kg) + curcumin (200 mg/kg) group. Then, 28-day-old female mice were divided into four groups: control group, curcumin (100 mg/kg) group, bisphenol A (10 mg/kg) group, and bisphenol A (10 mg/kg) + curcumin (100 mg/kg) group to explore the protective mechanism of curcumin. Corn oil, bisphenol A and/or curcumin were orally administered for six weeks. After treatment, some ovaries were collected to measure reactive oxygen species (ROS) levels and to observe follicular atresia by histological evaluation after hematoxylin-eosin staining. The mRNA expression and activities of antioxidants such as superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) were also tested. The experimental data were statistically analyzed with variance analysis. The results showed that Bisphenol A exposure increased reactive oxygen species levels and induced ovarian oxidative stress, while co-treatment with curcumin could reverse bisphenol A-induced up-regulation of reactive oxygen species (Table 2). Bisphenol A exposure increased the percentage of atretic antral follicles and co-treatment with curcumin decreased antral follicle atresia (Table 2). Bisphenol A decreased the expression and activities of three key antioxidants, SOD, GPx and CAT. Co-treatment with curcumin could reverse bisphenol A-induced down-regulation of expression and activities of SOD, GPx and CAT (Fig. 2, 3). Therefore, it is possible that bisphenol A induces damage to the ovaries by oxidative stress pathway, and curcumin can reverse the ovary toxicity of bisphenol A to some degree.