硫丹对小鼠红细胞免疫功能的影响

为了探讨有机氯农药硫丹对小鼠(Mus musculus)红细胞免疫功能的影响,设计了体内、外两组实验。体内实验:将40只小鼠随机分成4组,灌胃硫丹的量依次为:0、0.4、1.6、6.4mg/(kg·d)。灌胃25d后,取血测定红细胞的免疫功能。体外实验:将9只小鼠的红细胞分别与不同浓度的硫丹在体外培养,实验设空白对照组、溶剂丙酮组和4个不同浓度硫丹组,其6组实验所用硫丹的量依次为:0、0、5、10、20、40μg/ml。体外培养2h后,测定红细胞免疫粘附能力。结果表明,在活体实验中,随着硫丹浓度的增加,小鼠红细胞C3b受体花环率(ratio of C3b rosetting,C3bRR)明显下降,依硫丹灌胃浓度由低到高,其C3bRR依次为7.78%、6.80%、4.96%、4.33%;而循环免疫复合物花环率(ratio of immune complexes rosetting,ICRR)随着硫丹浓度升高而升高,分别为6.69%、6.31%、7.86%、9.42%。红细胞促NK细胞活性的功能在各组间没有显著差异。硫丹6.4mg/(kg·d)组小鼠红细胞对T淋巴细胞免疫粘附促进能力较其他3组明显降低。血浆中红细胞天然免疫促进因子活性在1.6mg/(kg·d)组和6.4mg/(kg·d)组较0mg/(kg·d)组明显降低。与溶剂丙酮组相比,血浆中红细胞天然免疫抑制因子活性在0.4mg/(kg·d)组明显下降,而在6.4mg/(kg·d)组却明显升高。离体红细胞经硫丹处理后其C3bRR显著降低,4个硫丹处理组依其浓度由低到高,C3bRR依次为:6.14%、5.56%、5.06%、4.44%;而ICRR却显著升高,分别为6.69%、6.31%、7.86%、9.42%。这表明,硫丹能抑制小鼠红细胞免疫粘附能力和红细胞对T淋巴细胞的正向调节功能,降低血浆中红细胞天然免疫促进因子活性,而对抑制因子活性影响比较复杂,低剂量时起抑制作用,而高剂量时能促进其活性。

Effect of Endosulfan on Immune Function of Erythrocytes in Mice

To investigate the effects of organ chlorine pesticide-on endosulfan on erythrocyte immune functions in mice,in vivo and in vitro experiments were designed.A total of 40 mice were randomly divided into 4 groups in in vivo experiment.They were garaged with endosuffan at levels of 0,0.4,1.6,and 6.4 mg/(kg·d)for 25 days,respectively.Blood was then collected to determine the immune functions of erythrocytes.In in vitro experiment, the erythrocytes from 9 mice were incubated with different concentrations of endosulfan: 0(control),0(acetone,dissolvent),5,10,20,and 40 μg/ml,respectively.The immune adherent capacity of the erythrocyte was determined after euhuring for 2 hours in vitro.In vivo experiment showed that the ratio of erythrocyte. C3b rosetting (C3bRR) significantly decreased, while the ratio of immune complexes rosetting(ICRR) increased with the increase of endosulfan dosage. There was no significant difference among the 4 groups in the activity of regulating NK cells by erythrocytes. The activity of regnlating T cell adhering function by erythrocytes in 6. 4 mg/(kg·d) group was significantly lower compared with other three groups. The activity of erythrocyte natural adhering enhancing factor in plasma remarkably reduced in 1.6 mg/(kg·d) group and 6.4 rag/(kg·d) group compared with that of the control group. The activity of erythrocyte natural adhering inhibitory factor decreased in 0. 4 me,/(kg·d) group, but increased in 6.4 rag/(kg·d) group. In vitro, the C3bBB of erythmcytes declined,while the ICBR ascended with the increase of endosulfan concentration. These results suggest that endosulfan inhibits the erythrocyte immune adherent function and the positive regulating function of T cells by erythrocytes,and decreases the immune-enhancing activity of erythrocyte. The results also show that the effects of endosulfan on inhibitory factor activity are complicated: it inhibits the activity of inhibitory factor at the low dosage, while improves the activity of inhibitory factor at the high dosage.