奥尼罗非鱼胃肠道中肥大细胞的分布特征

本研究采用改良甲苯胺蓝染色法探讨了奥尼罗非鱼(Oreochromis niloticus ♀×O. aureus♂)胃肠道肥大细胞的分布及其形态特点。结果发现,经甲苯胺蓝染色的肥大细胞其核着深蓝色,颗粒被染成紫红色,着色深浅不一。肥大细胞大小不一,形态各异,呈圆形、椭圆形或梭形、菱形,散在或集中分布在黏膜层固有膜和黏膜下层,尤其常见分布于小血管周围。经统计,肥大细胞在奥尼罗非鱼的胃、幽门盲囊、后肠、前肠、中肠的数量依次减少。胃和幽门盲囊内肥大细胞数量显著高于前肠和中肠(P0.05),与后肠无显著差异;前肠、中肠和后肠内肥大细胞数量并无明显差别。

Distribution Characteristics of Mast Cells in Gastrointestinal Tract of Tilapia Galilaea

Distribution and morphological characteristics of mast cells in gastrointestinal tract of Tilapia Galilaea were studied by modified toluidine blue staining. The results showed that the mast cells scattered or concentrated in the lamina propria mucosa and submucosa, especially around small blood vessels (Fig. 1). Mast cells varied in their sizes and shapes. They were round, oval, fusiform or rhombic in shape, and their nuclei were in dark blue when stained with toluidine blue, and the granules were in purple red with varying shades of color (Fig. 2). Statistical analysis (Table 1) by one-way ANOVA of SPSS 24.0 showed that the number of mast cells in stomach (9.7 ± 1.9), pyloric caecum (8.3 ± 2.8), hindgut (8.3 ± 1.8), foregut (6.3 ± 1.9) and midgut (5.1 ± 1.8) of Tilapia Galilaea decreased in turn. The number of mast cells in gastric and pyloric caecum was significantly higher than that in foregut or midgut (P < 0.05), but there was no significant difference from that in hindgut, and the number of mast cells showed no significant difference in the foregut, midgut and hindgut. In conclusion, mast cells were mainly distributed in the lamina propria and tunica submucosa of various gastrointestinal tissues, with different sizes and shapes, and the number of mast cells decreased successively in the order of stomach, pyloric caecum, hindgut, foregut and midgut.