| 吉富罗非鱼两种生长激素受体基因的分离及与增重相关的SNPs位点 |
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| 引用本文： | 阮瑞霞,俞菊华,李红霞,李建林,唐永凯,魏可鹏.吉富罗非鱼两种生长激素受体基因的分离及与增重相关的SNPs位点[J].动物学杂志,2011,46(3):37-46. |
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| 作者姓名： | 阮瑞霞 俞菊华 李红霞 李建林 唐永凯 魏可鹏 |
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| 作者单位： | 南京农业大学无锡渔业学院;中国水产科学研究院淡水渔业研究中心农业部淡水鱼类遗传育种和养殖生物学重点开放实验室; |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项资金(No.2011JBFC03,2009JBFB05); 农业部淡水生物多样性保护与利用重点开放实验室第七期开放课题(No.LFBU0706); 公益性行业(农业)科研专项经费项目(No.200903046-02) |
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| 摘 要： | 实验分离了吉富罗非鱼(Oreochromis niloticus)两种生长激素受体基因GHR1和GHR2的全长cDNA以及GHR1阅读框中外显子2～9、GHR2阅读框外显子2～8的DNA序列.在GHR1和GHR2分别找到6个和16个SNPs位点,其中只有2个位点在外显子部分.使用PCR-RFLP方法检测了5个家系共12...
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| 关 键 词： | 吉富罗非鱼 生长激素受体 单核苷酸多态性 增重 组织表达 |
| 收稿时间： | 2010/11/15 0:00:00 |
| 修稿时间： | 3/7/2011 12:00:00 AM |
Isolation of Two Growth Hormone Receptor Genes and SNPs Associated with Body Weight in GIFT Strain Tilapia Oreochromis niloticus |
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| RUAN Rui-Xi,YU Ju-Hu,LI Hong-Xi,LI Jian-Lin,TANG Yong-Kai and WEI Kei-Peng.Isolation of Two Growth Hormone Receptor Genes and SNPs Associated with Body Weight in GIFT Strain Tilapia Oreochromis niloticus[J].Chinese Journal of Zoology,2011,46(3):37-46. |
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| Authors: | RUAN Rui-Xi YU Ju-Hu LI Hong-Xi LI Jian-Lin TANG Yong-Kai and WEI Kei-Peng |
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| Institution: | Wuxi Fishery College, Nanjing Agricultural University, Wuxi 214081;Wuxi Fishery College, Nanjing Agricultural University, Wuxi 214081; Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Ministry of Agriculture,Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China;Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China;Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China;Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China;Wuxi Fishery College, Nanjing Agricultural University, Wuxi 214081 |
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| Abstract: | Growth hormone receptors(GHR) which located on the cell membrane is the only pathway for growth hormone(GH) to get into cells and affect the growth in animals,they play a vital important role on animal growth.Two cDNA sequences and DNA sequences of exon 2-9 of GHR1,exon 2-8 of GHR2 were isolated from GIFT strain(Genetically Improved Farmed Tilapia strain,Oreochromis niloticus) using RT-PCR and PCR in this study.Six and sixteen SNPs were identified on GHR1 and GHR2 respectively,only two of which were located... |
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| Keywords: | GIFT GHRs SNP Weight gain Tissues expression |
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