| 罗氏沼虾缅甸野生原种rDNA-ITS区序列特征 |
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| 引用本文： | 陈雪峰,杨国梁,王军毅,高强,宫金华,叶少群,张海鹏.罗氏沼虾缅甸野生原种rDNA-ITS区序列特征[J].动物学杂志,2011,46(3):19-27. |
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| 作者姓名： | 陈雪峰 杨国梁 王军毅 高强 宫金华 叶少群 张海鹏 |
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| 作者单位： | 浙江省淡水水产研究所;浙江南太湖淡水水产种业有限公司; |
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| 基金项目： | 浙江省重大科技专项项目(No.2007C12059); 科技部农业科技成果转化资金项目(No.2007GB2C200124) |
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| 摘 要： | 对罗氏沼虾(Macrobrachium rosenbergii)内转录间隔区(internal transcribed spacer,ITS)全序列特征进行了分析.罗氏沼虾ITS1长度为1 070～1 150 bp,GC含量为51.4%～52.7%;5.8S长度一致为163bp,GC含量为55.2%;ITS2长度为48...
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| 关 键 词： | 罗氏沼虾 内转录间隔区 序列特征 |
| 收稿时间： | 2010/10/14 0:00:00 |
| 修稿时间： | 3/2/2011 12:00:00 AM |
Analysis on Sequences of Ribosomal DNA Internal Transcribed Spacers of Macrobrachium rosenbergii |
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| CHEN Xue-Feng,YANG Guo-Liang,WANG Jun-Yi,GAO Qiang,GONG Jin-Hu,YE Shao-Qun and ZHANG Hai-Peng.Analysis on Sequences of Ribosomal DNA Internal Transcribed Spacers of Macrobrachium rosenbergii[J].Chinese Journal of Zoology,2011,46(3):19-27. |
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| Authors: | CHEN Xue-Feng YANG Guo-Liang WANG Jun-Yi GAO Qiang GONG Jin-Hu YE Shao-Qun and ZHANG Hai-Peng |
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| Institution: | Zhejiang Institute of Freshwater Fisheries, Huzhou 313001; Zhejiang South Tailake Freshwater Fish Breeding Co., Ltd, Huzhou 313001, China;Zhejiang Institute of Freshwater Fisheries, Huzhou 313001; Zhejiang South Tailake Freshwater Fish Breeding Co., Ltd, Huzhou 313001, China;Zhejiang Institute of Freshwater Fisheries, Huzhou 313001; Zhejiang South Tailake Freshwater Fish Breeding Co., Ltd, Huzhou 313001, China;Zhejiang Institute of Freshwater Fisheries, Huzhou 313001; Zhejiang South Tailake Freshwater Fish Breeding Co., Ltd, Huzhou 313001, China;Zhejiang South Tailake Freshwater Fish Breeding Co., Ltd, Huzhou 313001, China;Zhejiang South Tailake Freshwater Fish Breeding Co., Ltd, Huzhou 313001, China;Zhejiang South Tailake Freshwater Fish Breeding Co., Ltd, Huzhou 313001, China |
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| Abstract: | Internal transcribed spacers in ribosomal DNA were often used as molecular sequence in phylogenetic study.The complete sequence of internal transcribed spacers of Macrobrachium rosenbergii was isolated by PCR.The length of ITS1 was 1 070-1 150 bp with a GC content of 51.4%-52.7%,and the length of 5.8S was 163 bp with a GC content of 55.2%.Compared to ITS1,the length of ITS2(484-545 bp) was shorter while GC content(53.4%-54.6%) was higher.By comparison of sequence from different individuals,61 SNP loci were ... |
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| Keywords: | Macrobrachium rosenbergii Internal transcribed spacers(ITS) Sequence analysis |
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