准噶尔雅罗鱼组织同工酶表达特性

使用不连续PAGE法,分析了10尾准噶尔雅罗鱼(Leuciscus merzbacheri)眼睛、鳃、皮、背部肌肉、鳍和肝胰脏6种组织的10种同工酶(LDH,CCO,EST,CAT,POD,ME,MDH,G6PD,GDH,ADH)的差异表达,并对部分同工酶基因位点及表达酶谱表型进行了分析,以期为其种质资源保护和开发以及遗传育种等方面的研究提供基础资料。结果显示,10种同工酶中9种在6种组织中出现了明显的组织差异性,仅CCO在6种组织中的差异性较小。在对准噶尔雅罗鱼的10种同工酶的遗传多样性分析中,共记录到了21个基因位点,其中Est-1、Me-B、s-MDH、G6pd-A、G6pd-B和Adh-A为多态性基因位点。多态位点比例为:P=6/21=28.57%。

The Specific Expression of Isozymes in Leuciscus merzbacheri Tissues

Polyacrylamide gel electrophoresis was used to detect the expression of ten isozymes (LDH, CCO, EST, CAT, POD, ME, MDH, G6PD, GDH, and ADH) in six different tissues, including eye, gill, skin, muscle, fin, and liver. Part of isozymes gene loci and zymograms were analyzed for providing the foundation for conservation and development of germplasm resources and genetic breeding. The results showed that the electrophoresis patterns of isozymes exhibited an apparent tissue-specificity except for CCO which showed no evident difference in 6 tissues examined. Biochemical genetic analysis showed that the ten isozymes were coded by 21 gene loci, six of which were found to be polymorphic. Finally, based on the zymograms, six isozymes including Est-1, Me-B, s-MDH, G6pd-A, G6pd-B and Adh-A were analyzed genetically for their subunit structure, coding gene loci and numbers as well as their specific expression, and the proportion of polymorphic loci was P=6/21=28.57%.