白条草蜥消化道内分泌细胞的免疫组织化学

应用6种胃肠激素抗血清和免疫组织化学ABC法(avidin-biotin complex method),对白条草蜥(Takydromus wolteri)消化道内分泌细胞进行了免疫组织化学定位研究和形态学观察。结果表明,5-羟色胺细胞较其他5种内分泌细胞的分布更为广泛,整个消化道中(即从食管到直肠)均有分布,其分布密度高峰位于幽门。食管、回肠和直肠未检测到生长抑素细胞,生长抑素细胞在幽门部分布密度最高,总体来说生长抑素细胞的分布在胃部较高而在小肠部较低。胃泌素细胞和胰多肽细胞分布在小肠,均在十二指肠分布密度最高。胰高血糖素细胞在胃幽门部分布密度最高,十二指肠、空肠次之,回肠分布密度最低。P-物质细胞仅分布于幽门部。6种内分泌细胞以圆形和锥体形为主,它们广泛分布于消化道黏膜之间、腺泡上皮细胞之间及上皮细胞基部。内分泌细胞的密度分布与其食性、食物组成和生活环境有关,它们的形态与其内、外分泌功能是相适应的。

Immunohistochemical Studies on Endocrine Cells in the Digestive Tract of Takydromus wolteri

The distribution and density of endocrine cells in the digestive tract of Takydromus wolteri were studied by the method of ABC (avidin-biotin complex method) immunohistochemical technique with six gut hormone antisera.5-HT cells distributed throughout the digestive tract from esophagus to rectum,with the highest density in the pylorus.The other five kinds of endocrine cells did not distribute as widely as the 5-HT cells.SS cells were not detected in the esophagus,ileum and rectum.The density of SS cells was the highest in the pylorus,followed by stomach part,and they were rarely observed in small intestine.Gas cells and PP cells distributed in small intestine,with the highest density in the duodenum.The distribution density of Glu cells was the highest in the pyloricus,followed by duodenum and jejunum,the lowest in the ileum.SP cells were only found in the pylorus.The six kinds of endocrine cells were mainly in round shape and shuttled shape.They widely lied between epithelial cells,between glandular epithelial cells and at the bottom of epithelia.The distribution density of endocrine cells was related to its feeding habit,food component and living environment.And morphologies of endocrine cells were conformable with the endocrine and exocrine functions.