黑线姬鼠与大林姬鼠组织中超氧化物酶 和过氧化物酶的分布与活性比较

以黑线姬鼠(Apodemus agrarius)和大林姬鼠(A. peninsulae)为研究对象,采用聚丙烯酰胺凝胶电泳(PAGE)不连续体系的方法,比较分析了心、肝、肾、肌肉、脑、肺6种器官和组织中超氧化物酶(SOD)和过氧化物酶(POD)活性,并建立了2种酶的电泳图谱。结果显示,上述2种酶在黑线姬鼠和大林姬鼠的6种器官和组织中均有表达并表现出明显的特异性,其中,2种鼠中超氧化物酶共分离出迁移率由0.15~0.66的9条电泳谱带,过氧化物酶共分离出迁移率由0.09~0.83的20条电泳谱带。在肝和肺中酶的活性最强,黑线姬鼠6种器官和组织中超氧化物酶活性均强于大林姬鼠,2种鼠组织中过氧化物酶的活性和分布相似,但在同一物种不同器官和组织间过氧化物酶的活性及分布存在明显差异。

Distribution and Activity of Superoxide Dismutase and Peroxidase in Various Tissues of Apodemus agrarius and A. peninsulae

The discontinuous polyacrylamide gel electrophoresis (PAGE) was used to analyze and compare the activities of superoxide dismutase (SOD) and peroxidase (POD) in six tissues and organs including heart, liver, kidney, muscle, brain and lung of Apodemus agrarius and A. peninsulae. SOD and POD were expressed in all six tissues and organs analyzed, but both enzymes showed their specific characteristics in different tissues or organs of Apodemus agrarius and A. peninsulae. A total of 9 polyacrylamide gel electrophoresis bands with a mobility of 0.15 to 0.66 were separated for SOD, while 20 bands with a mobility of 0.09 to 0.83 were separated for peroxidase. Strongest activities of the two enzymes were found in the liver and lung. The activities of SOD in 6 tissues and organs of A. agrarius were stronger than in A. peninsulae. The activities and distribution of POD in the six tissues and organs of A. agrarius and A. peninsulae were similar. However, there were significant differences in POD activity and distribution among tissues and organs in the same species.