纳米硒通过抗氧化应激调节大脑NO含量改善睡眠剥夺小鼠认知功能

研究了纳米硒对睡眠剥夺(SD)小鼠(Mus musculus)认知功能的影响,并探讨其作用机制。将120只雄性昆明小鼠随机分成两批,第一批24只分为3组:对照组(NC)、亚硒酸钠组(SE)和纳米硒组(NS),分别给予硒浓度为4μg/ml的亚硒酸钠和纳米硒溶液每只0.5ml/d,NC组给等体积蒸馏水,连续30d,第31天测定SE和NS两组小鼠的血硒及全血GSH-Px活性,评价两种硒源的生物利用性;第二批96只分为4组:对照组(N-SeC),纳米硒低、中、高剂量组(L、M、H),L、M和H组分别给予硒浓度为2μg/ml、4μg/ml、8μg/ml的纳米硒溶液每只0.5ml/d,N-SeC组给予同体积蒸馏水,连续30d。第二批小鼠每组又各自分为4小组:SD对照组(SDC)及SD18h、SD36h、SD54h组,采用单平台水环境法(SPM)制作小鼠SD模型。在SD后,N-SeC、L、M和H组利用Y-型迷宫试验测定认知能力,同时测定小鼠大脑GSH-Px、NO、MDA含量。结果表明,纳米硒对GSH-Px活性的提高优于传统硒源亚硒酸钠,但血硒无显著差异;与SDC组比较,SD降低了小鼠的认知能力及大脑GSH-Px活性,提高了NO和MDA含量;与N-SeC比较,纳米硒使SD小鼠的认知功能得到改善,大脑GSH-Px活性提高,MDA和NO含量下降。上述结果表明,纳米硒能够改善SD小鼠的认知功能,这可能与其提高大脑GSH-Px活性并降低了自由基对大脑神经的损害有关。

Cognition Performance in Sleep-Deprived Mice is Improved by Cerebral NO Content Adjustment via Anti-Oxidation of Nano-Selenium

To study the effects and mechanisms of Nano-Se on cognition performance after sleep deprivation (SD) in mice.A total of 120 male mice were randomly divided into 2 batches.The first batch of 24 mice Was randomly divided into 3 groups:control (NC),Ne_2qSeO_3 (SE) and Nano-Se (NS).These mice were administered respectively with SE and NS (1 μ/d,2μg/d,4μg/d) by intra-gastric injection for 30 days,then their Se content and GSH.Px activity in blood were meaSured;The second batch of 96 mice were randomly divided into 4 large groups: control (N -SeC) and low, middle, high Nano-Se groups (L, M, H). Nano-Se solutions (2 μg/ml,4 μg/ml,8 μg/ml) were administered (0.5 ml;/d) to mice of L, M and H groups respectively by intra-gastric injection for 30 days,and the control group was administered with distilled water. Each large group was divided into 4 groups:SD control (SDC),SD18h,SD36h and SD54h. SD model was induced in mice by single platformtechnique. In the SD mice,the cognitive ability of the mice were tested by Y-maze. Further, brain GSH-Px, NO and MDA levels were measured. Blood GSH-Px activity was enhanced by Nano-Se compared to SE group, but the blood Se content was not different. SD decreased cognitive ability and GSH-Px activity, but increased NO andMDA levels. Compared to N-SeC, Nano-Se improved cognitive ability, increased GSH-Px activities, while decreased NO levels in M and H groups. MDA reduction was found in H group only. Our data suggest that Nano-Se could improve cognitive ability of mice,the mechanism of which might involve the increased GSH-Px activity and decreased free radical content.