高原鼢鼠寄生皮蝇的形态和分子鉴定

皮蝇蛆病是由双翅目(Diptera)狂蝇科(Oestridae)皮蝇亚科(Hypodermatinae)幼虫感染所导致的一种寄生虫病,会严重影响农牧业发展与公共卫生安全。本研究采用形态学和分子系统学方法对甘南藏族自治州碌曲境内高原鼢鼠(Eospalax baileyi)感染的皮蝇进行鉴定,旨在确定皮蝇的种类,为皮蝇蛆病预防、诊断和控制提供参考。结果发现,高原鼢鼠感染的皮蝇,其三龄幼虫的形态与兔裸皮蝇(Oestromyia leporina)三龄幼虫较为相似;皮蝇三龄幼虫COI基因序列与兔裸皮蝇的差异最小,平均差异为0.63%。系统进化树显示,感染高原鼢鼠的皮蝇三龄幼虫与兔裸皮蝇处于同一进化枝,其亲缘关系最近。因此,本研究确定甘南藏族自治州碌曲境内高原鼢鼠所感染的皮蝇为兔裸皮蝇。

Morphological and Molecular Identification of Torsalo Infecting Plateau Zokor (Eospalax baileyi)

Hypodermosis is a disease caused by the infection of Hypodermatinae (Diptera, Oestridae) larvae. It seriously impact the development of agriculture, stock-breeding and public health. In this study we used morphological and molecular methods to identify the torsalo species infecting Plateau Zokor (Eospalax baileyi) in Luqu county, Gannan Tibetan Autonomous Prefecture, Gansu and to provide correct taxonomy for prevention, diagnosis and control of Hypodermosis. The result showed that the morphological characters of the third instar larvae were similar to the third instar larvae of Oestromyia leporina (Table 1). The difference of the partial COI gene sequences between the third instar larvae of studied torsalo and O. leporina was the smallest when compared with other three species of torsalo, with an average difference of 0.63% (Table 2). Based on phylogenetic analysis, the genetic distance between third instar larvae of studied torsalo and O. leporina was the shortest (Fig. 4). Therefore, it was determined that the torsalo infecting plateau zokor in Luqu county of Gannan Tibetan Autonomous Prefecture was O. leporina.