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D-木糖对高脂血症大鼠脂代谢调节的作用机制
引用本文:何东,王晓雨,程玉刚,刘宛灵,丁继程,王向东,李霞.D-木糖对高脂血症大鼠脂代谢调节的作用机制[J].动物学杂志,2012,47(6):103-111.
作者姓名:何东  王晓雨  程玉刚  刘宛灵  丁继程  王向东  李霞
作者单位:山东大学医学院 济南 250012;山东大学医学院 济南 250012;山东大学医学院 济南 250012;山东大学医学院 济南 250012;济南圣泉唐和唐生物科技有限公司 济南 250100;山东大学医学院 济南 250012;山东大学医学院 济南 250012
摘    要:为了探讨D-木糖对于高脂血症模型大鼠(Rattus norregicus)脂代谢的调控作用及其分子机制,以高脂饲料饲喂Wistar雄性大鼠7周建立了高脂血症模型大鼠,以同批次饲喂普通饲料的大鼠作为正常对照组;后将高脂血症模型大鼠再分为4组,包括高脂血症模型组,高浓度D-木糖给药组(给药剂量按照大鼠的体重为1.2 mg/g·d),中浓度D-木糖(给药剂量0.6 mg/g·d)给药组,低浓度D-木糖给药组(给药剂量0.3 mg/g·d)。给药组以灌胃方式给药,正常对照组和高脂血症模型组动物同时灌胃等量生理盐水。6周后处死动物取材,计算脂/体比;肝冰冻切片观察肝的病理学变化;肝匀浆处理,以蛋白质印迹法测定高密度脂蛋白受体(HDL-R)的表达变化;同时测定相关血液生化指标。结果表明,高脂血症模型大鼠给予D-木糖干预后,各剂量给药组的体重与脂/体比显著低于高脂血症模型组(P<0.05),二者谷胱甘肽过氧化物酶、总脂酶表达差异显著(P<0.05),但胆固醇、甘油三酯、高密度脂蛋白、低密度脂蛋白的差异不显著(P>0.05);病理学改变表现突出,高浓度给药组肝细胞坏死与高脂模型组以及中低浓度给药组相比明显减轻,小叶结构完整,脂肪变程度轻,且较少炎性浸润;HDL-R蛋白表达水平在正常对照组与高脂血症模型有显著差异,各剂量给药组HDL-R水平呈现一定的药物浓度依赖性,高浓度给药组更接近于正常对照组。说明高脂血症大鼠经D-木糖治疗后,肝脂肪性变和肝细胞坏死程度减轻,肝细胞HDL-R的缺乏得到明显改善,证实D-木糖在改善高脂血症大鼠肝细胞损伤状况、保护肝组织结构与功能完整的过程中发挥调节作用。

关 键 词:D-木糖  高脂血症  高密度脂蛋白受体
收稿时间:7/3/2012 12:00:00 AM
修稿时间:2012/9/21 0:00:00

The Effect of D-xylose on Lipid Metabolism of Rats with Hyperlipemia and Its Related Mechanism
HE Dong,WANG Xiao-Yu,CHENG Yu-Gang,LIU Wan-Ling,DING Ji-Cheng,WANG Xiang-Dong and LI Xia.The Effect of D-xylose on Lipid Metabolism of Rats with Hyperlipemia and Its Related Mechanism[J].Chinese Journal of Zoology,2012,47(6):103-111.
Authors:HE Dong  WANG Xiao-Yu  CHENG Yu-Gang  LIU Wan-Ling  DING Ji-Cheng  WANG Xiang-Dong and LI Xia
Institution:School of Medicine, Shandong University, Jinan 250012;School of Medicine, Shandong University, Jinan 250012;School of Medicine, Shandong University, Jinan 250012;School of Medicine, Shandong University, Jinan 250012;Healtang Biotech Co., Ltd, Jinan 250100, China;School of Medicine, Shandong University, Jinan 250012;School of Medicine, Shandong University, Jinan 250012
Abstract:To investigate the effect of D-xylose on lipid metabolism of rats(Rattus norregicus)with hyperlipemia and its related molecular mechanism, high-fat diet was used to feed the Wistar male rats to generate the hyperlipemia model, and normal diet was used to feed the same batch rats as normal control group. Then all the rats with hyperlipemia were divided into four groups, i.e., high dose D-xylose interfering group (1.2 mg D-xylose per gram body weight per day), medium dose D-xylose interfering group (0.6 mg D-xylose per gram body weight per day), low dose D-xylose interfering group (0.3 mg D-xylose per gram body weight per day), and control hyperlipemia group. Intragastric administration of D-xylose was performed in interfering groups, while the normal control group and hyperlipemia control group were administrated with equivalent amount of normal saline. After 6 weeks, all the rats were sacrificed for obtaining testing samples. The body fat content was measured; a portion of liver samples was used for frozen section in order to survey the pathological changes and another portion of liver was homogenized to test the biochemical changes; Western blot was employed to check the HDL-R expression changes; while the blood samples were used to test the biochemical changes. After D-xylose treatment of rats with hyperlipemia, significant difference was observed in the body weight and the ratio of body weight to fat tissue weight between each drug treatment group and hyperlipemia model group (P<0.05).The expression levels of GSH-Px, LPL and HL were significantly different among these groups (P<0.05), but the total cholesterol, low-density lipoprotein, high-density lipoprotein and triacylglycerol in rats treated with D-xylose were not reduced significantly (P>0.05). Pathological features in the group treated with high concentration of D-xylose was evidently eased than in hyperlipemia model group, medium or low dose treatment group: liver cell necrosis was reduced significantly, the hepatic lobule was complete, steatosis and inflammatory infiltration were mild. HDL-R protein expression level showed significant difference between normal control group and hyperlipemia model group, while HDL-R expression in the group treated with a high concentration of D-xylose was closer to the control group. Therefore, after the treatment of D-xylose, liver fatty change and liver necrosis of the rats with hyperlipemia have been alleviated, and the deficiency of HDL-R in liver cells has been improved. All the results indicate that D-xylose plays an important role in improving the damage of hyperlipemia to liver cells and organ function in rats.
Keywords:D-xylose  Hyperlipidemia  High density lipoprotein receptor
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