| 乌鳢三倍体诱导及其生长 |
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| 引用本文： | 邹远超,李中,李艳,覃川杰,岳兴建,文正勇,王淯,齐泽民,谢碧文.乌鳢三倍体诱导及其生长[J].动物学杂志,2017,52(1):75-84. |
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| 作者姓名： | 邹远超 李中 李艳 覃川杰 岳兴建 文正勇 王淯 齐泽民 谢碧文 |
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| 作者单位： | 内江师范学院生命科学学院,长江上游鱼类资源保护与利用四川省重点实验室 内江,641112 |
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| 基金项目： | 四川省科技厅应用基础项目（No. 2015JY0262），四川省大学生创新项目（No. 201410640002）； |
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| 摘 要： | 采用热休克抑制第二极体排放的方法诱导乌鳢(Channa argus)三倍体,以探索人工诱导乌鳢三倍体的理想热休克条件。采用DNA含量测定法和红细胞核大小鉴定法对获得的鱼苗倍性进行鉴定,同时对普通二倍体乌鳢群体与三倍体群体的生长差异进行了比较研究。结果表明,(1)热休克法适宜诱导条件为,(28±0.5)℃培育水温授精后4 min,在水温42℃条件下持续处理3 min,三倍体诱导率最高,达到87.69%;(2)三倍体与二倍体DNA含量差异极显著(P0.01),其比值为1.50︰1.00;(3)三倍体和二倍体在红细胞核长径、红细胞核体积、红细胞核面积等6项指标上存在极显著差异(P0.01);与二倍体相比,三倍体红细胞体积和核体积分别是其1.69倍和1.60倍;(4)在4月龄与8月龄,三倍体的体长和体重比二倍体稍高,但两者差异不显著(P0.05)。本实验结果为进一步开展乌鳢倍性育种奠定了基础。
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| 关 键 词： | 乌鳢 三倍体 诱导 热休克 生长 |
| 收稿时间： | 2016/1/19 0:00:00 |
| 修稿时间： | 2016/10/12 0:00:00 |
Induction and Growth of Triploid Snakehead (Channa argus) |
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| ZOU Yuan-chao,Li Zhong,QIN Chuan-Jie,YUE Xing-Jian WEN,WEN Zheng-Yong,WANG Yu,QI Ze-Min and XIE Bi-Wen.Induction and Growth of Triploid Snakehead (Channa argus)[J].Chinese Journal of Zoology,2017,52(1):75-84. |
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| Authors: | ZOU Yuan-chao Li Zhong QIN Chuan-Jie YUE Xing-Jian WEN WEN Zheng-Yong WANG Yu QI Ze-Min and XIE Bi-Wen |
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| Institution: | College of Life Sciences, Neijiang Normal University; Conservation and Utilization of Fishes resources in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province,,College of Life Sciences, Neijiang Normal University; Conservation and Utilization of Fishes resources in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province,,College of Life Sciences, Neijiang Normal University; Conservation and Utilization of Fishes resources in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province,,College of Life Sciences, Neijiang Normal University; Conservation and Utilization of Fishes resources in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province,,College of Life Sciences, Neijiang Normal University; Conservation and Utilization of Fishes resources in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province,,College of Life Sciences, Neijiang Normal University; Conservation and Utilization of Fishes resources in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province,,College of Life Sciences, Neijiang Normal University; Conservation and Utilization of Fishes resources in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province,,College of Life Sciences, Neijiang Normal University; Conservation and Utilization of Fishes resources in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, |
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| Abstract: | |
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| Keywords: | Snakehead (Channa argus) Triploidy Induction Heat shock Growth |
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